Human post-mortem nuclei preparation and single-nucleus multiome DNA/RNA sequencing by ResolveOME

By Ester Kalef-Ezra, PhD, George Morrow, Yanping Guo and Christos Proukakis, PhD
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Output Details

Description
Here we describe a protocol for nuclei isolation, immunostaining and sorting (FANS) for single-nucleus DNA/RNA-seq using ResolveOME (BioSkryB) method from human post-mortem brain samples. We have used it to isolate nuclei from human post-mortem substantia nigra tissue, but it can be adapted for nuclei from different body areas.The ResolveOME protocol is generally designed to work with single live mammalian cells and here we have done minor modifications in order to use it for human post-mortem brain samples.
Identifier (DOI)
10.17504/protocols.io.3byl498bzgo5/v1
Team
Team Voet
Program
Collaborative Research Network
Theme
PD Functional Genomics

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