Seeded amplification assay (SAA) from neuronal cell or postmortem brain lysates
By onDetection of minute amounts of misfolded α-synuclein aggregates in neuronal cell lysates.
Seeded amplification assay (SAA) from neuronal cell lysates
By onSeeded amplification assay (SAA) from neuronal cell lysates
Ultra-high field, multi-echo MRI acquisition for resting-state functional connectivity
By onMRI acquisition sequence at USC's Center for Image Acquisition examines motor circuits in Parkinson’s Disease using a Siemens 7T Terra scanner. Scans include anatomical T1 MPRAGE, spin-echo EPI, and multi-echo EPI sequences.
Proximity ligation assay
By onProximity ligation assay (PLA) in iPSC-derived cortical neurons. Permeabilization with Saponin detergent is used to preserve lipid-rich inclusions.
Seahorse XF Cell Mito Stress Test
By onSeahorse XF Cell Mito Stress Test for assessment of oxygen consumption rate in iPSC-derived neurons as a measure of mitochondrial respiration.
Quantitative PCR for STMN2 expression in pi-N neurons
By onQuantitative PCR for STMN2 expression in pi-NSNCA-STMN2 neurons.
Western blot of amplified fibrils after Proteinase K digestion
By onProtocol for western blot of amplified fibrils of a-synuclein after proteinase K digestion.
Mitochondrial subunit assay in hiPSC-derived neurons.
By onAssessment of mitochondrial subunit levels in hiPSC-derived neurons.
Step-centrifugation Assay
By onThis protocol details the protocol for conducting a step-centrifugation assay to determine the minimum required speed for full separation of vesicles from native nanodiscs.
Protocols: Phosphoglycerate kinase is a central leverage point in Parkinson’s Disease driven neuronal metabolic deficits
By onThe current collection contains all experimental protocols associated with the study "Phosphoglycerate kinase is a central leverage point in Parkinson’s Disease driven neuronal metabolic deficits".
Native Nanobleach of native nanodiscs
By onThis is a protocol to conduct Native Nanobleach of native nanodiscs.
LC-MS of Native Nanodiscs
By onThis protocol provides a step-by-step guide for conducting LC-MS analysis of native nanodiscs extracted from biological membranes.
Mitochondrial antigen presentation (MitAP) to primary 2C CD8 T (proliferation and suppression) assay
By onThis protocol details the mitochondrial antigen presentation (MitAP) to primary 2C CD8 T (proliferation and suppression) assay in vivo
Brain infiltrating leukocytes (BILs) extraction
By onThis protocol details the extraction of brain infiltrating leukocytes (BILs).
Intracranial injections of viral vectors in mouse striatum
By onProtocol for intracranial viral vector injections in dorsal or ventral striatum in mice is outlined. Steps are generalizable, but require optimization for injection coordinates and virus concentration.
Designing sgRNA Oligos and Inserting Guides into the GEARBOCS Vector
By onProtocol for designing sgRNAs for genes using GEARBOCS vector backbone enabling astrocyte-specific genetic manipulation in CRISPR/Cas9 mice for in vivo assays.
Protocol collection associated with “Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons”
By onThe protocol collection provides a toolkit for profiling endolysosomes in human induced neurons and hESCs using Endo-IP and Lyso-IP techniques.
Protocol collection for “Global cellular proteo-lipidomic profiling of diverse lysosomal storage disease mutants using nMOST”
By onProtocol collection for "Global cellular proteo-lipidomic profiling of diverse lysosomal storage disease mutants using nMOST"
Assessment of mouse motor behaviour via open field test, narrowing beam assay, inverted wire hang, and gait analysis
By onProtocol for assessing mouse behaviour on 4 tasks: open field test, narrowing beam assay, inverted wire hang, and gait analysis
Controls, imaging, and analysis of AAV-Zombie and SpECTr experiments
By onProtocol describing best practices for designing, imaging, and analyzing AAV-Zombie and SpECTr experiments
