Bioluminescence Imaging
By onThis protocol describes a bioluminescence stepwise imaging protocol in mice with AAV-luciferase expression in the vagal afferents using the Ivis Lumina series III imaging system.
Mitochondrial Antigen Presentation (MitAP) to 2CZ CD8+ T cell hybridoma
By onThis protocol details the Mitochondrial Antigen Presentation (MitAP) to 2CZ CD8+ T cell hybridoma
Preparation of qPCR standards for AAV titering
By onqPCR standards for AAV titering- document annex to protocol "AAV Purification Protocol with Iodixanol gradient"
Photometry Acquisition in Freely Moving Mice
By onThis protocol describes the steps for acquiring photometry data for freely moving mice.
Ex vivo electrophysiology
By onProtocol outlines ex vivo electrophysiology in mouse brain slices with guidance for clozapine N-oxide (CNO) testing in DREADD-expressing neurons.
Validating Responsiveness of AAV-DIO-hM3Dq-DREADD
By onProtocol for validating behavioral response to IP injection of clozapine-n-oxide in DAT-IRES-Cre mice injected with AAV-DIO-hM3Dq-DREADD in midbrain.
Human post-mortem nuclei preparation and single-nucleus multiome DNA/RNA sequencing by ResolveOME
By onProtocol for isolating nuclei, immunostaining, and sorting for single-nucleus DNA/RNA-seq using ResolveOME method is described for post-mortem brain samples.
Startle induced negative geotaxis (SING)
By onThis protocol describes how to perform the Startle induced negative geotaxis (SING) assay. SING defects in parkinsonism fly models have previously been shown to be caused by dopaminergic impairments.
Baseplate Implantation for Two-Part Chamber System
By onProtocol details implanting a baseplate onto the skull as the first step in creating a chamber system in rhesus macaques. The thin baseplate is enclosed subcutaneously to promote healing and osseointegration without infection risks.
Chronic Multi-Electrode Assembly Implantation with a Chamber Setup
By onProtocol for implanting carbon fiber microelectrodes for chronic recording using a two-part chamber system in rhesus macaques. Prior steps for chamber implantation are detailed in other protocols.
Craniotomy, and Second and Third Phase Implantation to Complete Two-Part Chamber System
By onThis protocol describes how to install the second or third phase of the two-part chamber system in rhesus macaques, as well as the craniotomy.
AAV Production and Purification
By onProtocol for producing and purifying Adeno-Associated Virus from HEK293T cell cultures is outlined
DAB Staining for Tyrosine Hydroxylase (TH) on Free-floating Fixed NHP Brain Tissue
By onThis protocol details immunohistochemical 3,3’-Diaminobenzidine (DAB) staining for Tyrosine Hydroxylase (TH) of free-floating fixed brain tissue sections using the avidin/biotin ABC complex and the Rabbit Polyclonal Anti-TH NB300-109 (Novus).
Direct ELISA
By onThe protocol explains how to perform a direct ELISA on a specific protein of interest, outlining the setup and running procedures.
Targeted optogenetic stimulations
By onThis protocol includes the steps for targeted optogenetic manipulation using a new micro-fiber array approach that measures and optogenetically manipulates local dynamics across multiple targeted locations simultaneously in behaving mice.
Stereotaxic viral injections and array implantation
By onThis protocol includes the stereotaxic viral injections and array implantation steps using a micro-fiber array approach that measures and optogenetically manipulates local dynamics across multiple targeted locations simultaneously in behaving mice.
Freely moving imaging
By onThis protocol includes the steps for imaging in freely-moving mice using a new micro-fiber array approach that measures and optogenetically manipulates local dynamics in multiple sites.
Head-fixed behavior
By onThis protocol includes the steps for imaging in head-fixed mice using a new micro-fiber array approach that measures and optogenetically manipulates local dynamics in multiple sites.
DFP-induced mitophagy experiments
By onThis protocol details how to induce mitophagy with Deferiprone (DFP).
ATG9A-vesicle isolation protocol
By onThis protocol details the ATG9-vesicle isolation procedure from Hap1 cells.
