Motor Behavioral Assessment
By onProtocol outlines motor behavior tests for Parkinson's disease in mice using open field, rotarod, cylinder, and balance beam tests. Mice were habituated for 30-40 min before tests conducted 3-4 weeks post-injections.
Electrophysiology and 2-photon imaging of Ca+2-transients
By onProtocol for imaging dendritic calcium transients using 2-photon microscopy with a Ti:Sapphire laser. Fluorescence collected through objective, dichroic mirror, and filters, then detected by a GaAsP PMT. Output digitized for analysis.
Ex Vivo Electrophysiology – Ford Lab
By onThis protocol describes the steps to perform whole-cell electrophysiology recordings in acute brain slices.
Behavioral tasks-Falasconi & Kanodia et al 2025
By onProtocol for freely moving pellet or water reaching, lever pressing and open field behavioral assays from Falasconi & Kanodia et al 2025.
Lysosomal GCase (glucocerebrosidase) activity assay
By onMethod to measure lysosomal GBA1 enzyme activity using 4-MUG substrate at low pH to minimize non-lysosomal GCase interference. GBA1 activity higher in purified lysosomes than whole cell extract, confirmed by CBE inhibitor abolishing 4-MUG hydrolysis.
Flowcytometry analysis of lysosomal pulldown with anti-TMEM192 magnetic beads from PBMCs
By onMethod described for staining intact lysosomes with lysotracker for flow cytometry. Analysis provides quantitative indication of organelle enrichment in immunoprecipitation, serving as quality control.
Dephosphorylation of phosphorylated Rab GTPases by PPM phosphatases
By onProtocol measures phosphoRab GTPase dephosphorylation with PPM phosphatases using purified phosphoRab GTPases. Substrates generated by phosphorylating Rab GTPases with MST3 kinase are included.
Phosphatome-wide siRNA screen in 3T3 cells
By onProtocol details a phosphatome-wide siRNA screen in 3T3 cells to identify phosphatases counteracting LRRK2 for pRab12 and pRab10.
GCase assay – Resorufin β-D-glucopyranoside
By onMethod presented for assessing lysosomal enzyme GBA1 activity using Resorufin substrate in low pH conditions to minimize non-lysosomal activity. Validated in GBA1 KO cells, compatible with various lysates and adjustable for different GBA1 levels.
FISH CODETECTION
By onDIG and Fluorescein labeled riboprobes Fluorescent in situ hybridization Frozen brain sections
Protocols for Goldman et al. 2025
By onAll protocols related to GEM-SCOPe and described in Glodman et al. 2025
Image Processing and 3D Reconstruction – Chen et al. 2025 NSMB
By onImage processing and 3D reconstruction applied in Chen et al. 2025 NSMB
In vivo voltammetry and fiber photometry in the mouse striatum
By onProtocol measures dopamine and calcium levels in striatal dopaminergic axons in vivo via voltammetry and fiber photometry in response to electrical stimulation.
Determining the effects of mecamylamine in the mouse striatum using a Conditioned Preference Place (CPP) paradigm
By onProtocol tests mecamylamine effects in mouse striatum using CPP paradigm to measure preference for drug-associated environment.
Intracranial injections of viral vectors in mouse midbrain and striatum
By onProtocol for injecting viral vectors in mouse midbrain and striatum is provided.
Aspiration of Fluid in Semi-Sealed Chamber
By onProtocol for post-op maintenance of electrode chambers emphasizes fluid aspiration and contamination prevention to reduce infection risk. Regular cleaning is crucial for asepsis and preventing complications like meningitis.
s-µIP Fabrication Process
By onThis protocol describes the fabrication of s-µIP microelectrodes. These devices are made of either one or two parylene-coated tungsten-carbon fiber assemblies, threaded through a silica tube.
Fast-scan cyclic voltammetry to assess dopamine release in ex vivo mouse brain slices following electrical or optogenetic stimulations
By onThis protocol is to assess changes in extracellular dopamine concentration following electrical or optogenetic stimulations in ex vivo mouse brain slices.
