LC-MS of Native Nanodiscs
By onThis protocol provides a step-by-step guide for conducting LC-MS analysis of native nanodiscs extracted from biological membranes.
Brain infiltrating leukocytes (BILs) extraction
By onThis protocol details the extraction of brain infiltrating leukocytes (BILs).
Mitochondrial antigen presentation (MitAP) to primary 2C CD8 T (proliferation and suppression) assay
By onThis protocol details the mitochondrial antigen presentation (MitAP) to primary 2C CD8 T (proliferation and suppression) assay in vivo
Intracranial injections of viral vectors in mouse striatum
By onProtocol for intracranial viral vector injections in dorsal or ventral striatum in mice is outlined. Steps are generalizable, but require optimization for injection coordinates and virus concentration.
Designing sgRNA Oligos and Inserting Guides into the GEARBOCS Vector
By onProtocol for designing sgRNAs for genes using GEARBOCS vector backbone enabling astrocyte-specific genetic manipulation in CRISPR/Cas9 mice for in vivo assays.
Protocol collection associated with “Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons”
By onThe protocol collection provides a toolkit for profiling endolysosomes in human induced neurons and hESCs using Endo-IP and Lyso-IP techniques.
Protocol collection for “Global cellular proteo-lipidomic profiling of diverse lysosomal storage disease mutants using nMOST”
By onProtocol collection for "Global cellular proteo-lipidomic profiling of diverse lysosomal storage disease mutants using nMOST"
AAV-Zombie on cultured cells
By onProtocol for detecting AAV genomes and concatemers in cultured cells by the Zombie method
Immunohistochemistry (IHC) on mouse brain slices
By onProtocol for immunohistochemical localization and visualization of proteins in mouse brain tissue (free-floating or slide-mounted)
Controls, imaging, and analysis of AAV-Zombie and SpECTr experiments
By onProtocol describing best practices for designing, imaging, and analyzing AAV-Zombie and SpECTr experiments
AAV-Zombie on tissue sections
By onProtocol for detecting AAV genomes and concatemers in tissue by the Zombie method
Assessment of mouse motor behaviour via open field test, narrowing beam assay, inverted wire hang, and gait analysis
By onProtocol for assessing mouse behaviour on 4 tasks: open field test, narrowing beam assay, inverted wire hang, and gait analysis
NanoString nCounter Gene Expression CodeSet RNA Hybridization Protocol
By onNanoString's technology uses molecular barcodes for digital detection in highly multiplexed analysis. Reporter CodeSet and Capture ProbeSet bind to targets, excess probes are removed, and barcodes are counted on nCounter™ systems.
Rating of levodopa-induced dyskinesia in 6-OHDA lesioned mice
By onThis protocol describes scoring of abnormal involuntary movements (AIMs), the behavioral manifestation of levodopa-induced dyskinesia, in 6-OHDA lesioned mice.
