Assay for lysosomal activity using Magic Red
By onAssay for lysosomal Cathepsin B activity in neurons using Magic Red
Immunoprecipitation from primary neurons
By onProtocol for immunoprecipitating proteins from neuronal lysates
all protocols related to ‘Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and Parkinsonian motor deficits’
By onall protocols related to 'Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and Parkinsonian motor deficits
Choice Reaction Time Reaching Task for Non-human Primate
By onThis protocol describes the behavioral task used with both healthy and MPTP-induced hemiparkinsonian non-human primates in the Turner Lab at the University of Pittsburgh, as well as the training procedure for introducing the task to naïve NHPs.
Stereotaxic injections of viral vectors and chronic optical fiber implants in mouse brains
By onProtocol details surgical steps for viral injections and optical fiber implantation in mouse striatum to monitor neurotransmitter dynamics during learning and extinction, using genetic sensors and tetanus toxin to suppress acetylcholine release.
Multi-fiber photometry in head-fixed mice performing a dual-cue delay Pavlovian association
By onProtocol describes dual-cue Pavlovian association & multi-fiber photometry in head-fixed mice. Mice trained to link cues with rewards & undergo extinction phase with reduced rewards.
Axonal proteome analysis and Western Blot via APEX2 labeling
By onAxonal proteome analysis and western blot via APEX2 labeling related to Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
Drugs and treatment dosing
By onDrugs and treatment dosing protocol related to LRRK2 mediates haloperidol-induced changes in indirect pathway striatal projection neurons
Experiments in HEK-293 cells
By onProtocols related to experiments in HEK-293 cells in Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons preprint
Western blot and Phostag gels
By onWestern blot and Phostag gels protocol related to Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
Liquid Chromatography-Mass Spectrometry (LCMS) with striatal synaptosomes
By onLiquid Chromatography-Mass Spectrometry (LC-MS) with striatal synaptosomes assay related to Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons preprint
Intracellular ROS measurement in iPSC-derived microglia
By onIntracellular ROS measurement in iPSC-derived microglia
Cytokine Array
By onThe EMD MILLIPLEX‱ MAP Human Cytokine/Chemokine/Growth Factor Pane A – Immunology Multiplex Assay (Merck Millipore) is employed to simultaneously analyse TNF-α, IL-1β, IL 6, and INFγ with Bead-Based Multiplex Assay using the Luminex‱ technology.
