RNA Isolation Using QIAzol-RNeasy Mini Kit and cDNA Synthesis
By onProtocol for RNA isolation using QIAzol and RNeasy Mini columns, followed by cDNA synthesis with High-Capacity cDNA Reverse Transcription Kit. Covers cell harvesting, lysis, purification, and RNA quality for gene-expression studies.
Protocol for Lysosomal Hexose Enrichment in SH-SY5Y Cells
By onProtocol enriches hexoses in lysosomes of SH-SY5Y cells by serum starvation, sucrose loading, and invertase hydrolysis.
