Immunofluorescencent Staining of A-synuclein, TFEB, GCase and LAMP1 in treated cultured cells
By onProtocol used to detect a-synuclein, TFEB, GCase, and LAMP1 via immunofluorescence in SH-SY5Y cells with mutant SNCA gene.
Isometric forelimb task behavioral training
By onThis task is a self-paced task in which mice are reinforced for using their forelimb to apply force to an immobile joystick exceeding a threshold of duration and force.
EMG recordings-Rodrigues-Vaz and Athalye et al, 2025
By onThis is the standard protocol for recordings of bulk EMG used for Rodrigues-Vaz and Athalye et al, 2025.
SDS and Clear Native Gel Electrophoresis
By onProtocol outlines SDS-PAGE & clear native PAGE for analyzing α-synuclein, Gba, & related proteins from mouse brain samples.
α-Synuclein binding assay to isolated synaptic vesicles
By onBinding of α-synuclein to synaptic vesicles was tested using His-tagged α-syn and Co Dynabeads. Mouse brain synaptic vesicles were isolated, incubated with α-syn, and analyzed using immunoblotting.
Measurement of Lysosomal pH Using LysoSensor Yellow/Blue DND-160
By onProtocol measures lysosomal pH with LysoSensor Yellow/Blue DND-160 dye. Steps include dye incubation, fluorescence reading, and pH calibration curve for accurate pH values.
Co-Immunoprecipitation (co-IP) Protocol
By onCo-IP is a common method to study protein-protein interactions in cells.
Magic Red® Cathepsin B Assay Protocol
By onThe Magic Red Cathepsin B Assay Kit detects intracellular cathepsin B activity in live cells using a fluorescence-based assay with a red fluorescent signal. It utilizes a specific substrate that releases a fluorophore when cleaved by cathepsin B.
ROS Measurement Using CellROX
By onProtocol uses CellROX fluorescent probe to measure cellular oxidative stress via flow cytometry.
Assessment of Mitochondrial OCR For SH-SY5Y Cell using Agilent Seahorse XFe Cell Mito Stress Test
By onProtocol assesses mitochondrial function in SH-SY5Y cells using Agilent Seahorse XFe Cell Mito Stress test measuring OCR. It also examines impact of iron supplementation and ROS inhibition on mitochondrial function.
Flow Cytometry Protocol for LAMP1 Staining V.2
By onProtocol details staining of LAMP1 in human cells using flow cytometry.
Protocols for “LRRK2 G2019S mutation incites increased cell-intrinsic neutrophil effector functions and intestinal inflammation in a model of infectious colitis.”
By onProtocols for "LRRK2 G2019S mutation incites increased cell-intrinsic neutrophil effector functions and intestinal inflammation in a model of infectious colitis."
RNA Isolation Using QIAzol-RNeasy Mini Kit and cDNA Synthesis
By onProtocol for RNA isolation using QIAzol and RNeasy Mini columns, followed by cDNA synthesis with High-Capacity cDNA Reverse Transcription Kit. Covers cell harvesting, lysis, purification, and RNA quality for gene-expression studies.
Protocol for Lysosomal Hexose Enrichment in SH-SY5Y Cells
By onProtocol enriches hexoses in lysosomes of SH-SY5Y cells by serum starvation, sucrose loading, and invertase hydrolysis.
