Immunofluorescence and confocal microscopy
By onThis protocol details the process of immunofluorescence and confocal microscopy.
Kinase activity assays Src and CK2
By onThis protocol details about the Kinase activity assays of Src and CK2.
Fast-scan cyclic voltammetry to assess dopamine release in ex vivo mouse brain slices while optogenetically activating astrocytes
By onThis protocol is to assess whether optogenetically activating astrocytes affects the dopamine concentration released following electrical stimulation.
Western blot: Alpha-synuclein
By onProtocol for detecting alpha-synuclein protein in mouse medulla oblongata tissue via western blot is outlined.
Western Blot Analysis V.1 used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By onWestern Blot Analysis V.1 was utilized in a study focusing on how LRRK2-G2019S, aging, and inflammation impact gut and peripheral immune cell activation before nigrostriatal degeneration occurs.
List of IHC/WB antibodies, RT-PCR probes and software used
By onList of IHC/WB antibodies, RT-PCR probes and software used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration"
Luminex-Based Multiplex Cytokine Analysis protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By onLuminex-Based Multiplex Cytokine Analysis protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration"
ADP Glo Protocol
By onProtocol for ADP Glo (Promega) assay to measure the catalytic activity of the lipid kinase VPS34 on small unilammelar vesicles.
Ultra-high field, multi-echo MRI acquisition for resting-state functional connectivity
By onMRI acquisition sequence at USC's Center for Image Acquisition examines motor circuits in Parkinson’s Disease using a Siemens 7T Terra scanner. Scans include anatomical T1 MPRAGE, spin-echo EPI, and multi-echo EPI sequences.
Seeded amplification assay (SAA) from neuronal cell lysates
By onSeeded amplification assay (SAA) from neuronal cell lysates
Seeded amplification assay (SAA) from neuronal cell or postmortem brain lysates
By onDetection of minute amounts of misfolded α-synuclein aggregates in neuronal cell lysates.
Western blot of amplified fibrils after Proteinase K digestion
By onProtocol for western blot of amplified fibrils of a-synuclein after proteinase K digestion.
Seahorse XF Cell Mito Stress Test
By onSeahorse XF Cell Mito Stress Test for assessment of oxygen consumption rate in iPSC-derived neurons as a measure of mitochondrial respiration.
Quantitative PCR for STMN2 expression in pi-N neurons
By onQuantitative PCR for STMN2 expression in pi-NSNCA-STMN2 neurons.
Proximity ligation assay
By onProximity ligation assay (PLA) in iPSC-derived cortical neurons. Permeabilization with Saponin detergent is used to preserve lipid-rich inclusions.
Mitochondrial subunit assay in hiPSC-derived neurons.
By onAssessment of mitochondrial subunit levels in hiPSC-derived neurons.
Protocols: Phosphoglycerate kinase is a central leverage point in Parkinson’s Disease driven neuronal metabolic deficits
By onThe current collection contains all experimental protocols associated with the study "Phosphoglycerate kinase is a central leverage point in Parkinson’s Disease driven neuronal metabolic deficits".
Step-centrifugation Assay
By onThis protocol details the protocol for conducting a step-centrifugation assay to determine the minimum required speed for full separation of vesicles from native nanodiscs.
Native Nanobleach of native nanodiscs
By onThis is a protocol to conduct Native Nanobleach of native nanodiscs.
