Cathepsin D assay to verify the retention of lysosomal content
By onHere, the authors describe a method where the measurement of cathepsin D activity is used to verify the intactness of lysosomes.
Protocol for performing PINK1 siRNA knockdown in mouse embryonic fibroblasts (MEFs)
By onThis protocol details the siRNA knockdown in mouse embryonic fibroblasts (MEFs) for PINK1, but applicable for any other target.
Immunostaining and quantification of intracellular accessible cholesterol using ALOD4-mNeon in Human Fibroblasts
By onProtocol for quantifying intracellular cholesterol in human fibroblasts using ALOD4-mNeon presented.
Immune stimulation of human induced pluripotent stem cells (hiPSC)-derived glia with lipopolysaccharide (LPS)
By onhiPSCs are used to model human development and diseases. Lipopolysaccharide activates immune cells and triggers cytokine secretion. LPS activates toll-like receptor 4 and NFkB. Protocol how to use LPS to activate iPSC-derived microglia.
Production of α-synuclein preformed fibrils (PFF)
By onThis protocol outlines the procedure to produce preformed fibrils (PFF). It has been adapted from Volpicelli-Daley et al., 2014.
Magic Red Cathepsin B activity assay
By onProtocol outlines red cathepsin B activity assay for cultured cells.
Lysosomal Membrane Permeability (LMP) assay
By onProtocol outlines LMP assay post cell treatment and fixation for assessing lysosomal membrane permeability.
Lysosomal activity DQ-Red BSA assay
By onProtocol outlines DQ-Red BSA Assay for live cell treatment and fixation for imaging lysosomal activity.
In Vitro αS PFF Treatment
By onProtocol outlines preparation of alpha synuclein preformed fibrils for in vitro cell treatment to study protein uptake, processing, and clearance.
Operant Behavior
By onThis protocol is for training mice operant conditioning to assess cognitive performance using the resources described in Matikainen-Ankney BA et al 2021.
Rotarod Test
By onBehavioral assay to measure motor impairments, specifically balance and coordination, in mice.
Open Field using Ethovision
By onProtocol for general locomotion in rodents using open field arenas and Ethovision video tracking. Purpose: Assess cognitive, spontaneous motor activity, and anxiety
Apomorphine-induced rotations
By onTo test for 6-OHDA lesion efficiency, rotational behaviors are assessed while mice are under the effects of apomorphine. This test is run during the dark phase of light cycle.
Isolation of brain infiltrating lymphocytes
By onThis protocol details the isolation of brain infiltrating lymphocytes after adoptive transfer of mitochondrial antigen- specific T cells.
Food intake behavior protocol
By onThis protocol describes the behavioral test to measure food intake in mice.
