Analysis of glycosphingolipids from animal tissues
By onInterest in the role of cellular glycosphingolipids (GSLs) in health and disease led the authors to develop a sensitive method to analyze the full complement of GSL structures present in mammalian cells, fluids, and tissues.
Purification of Total RNA from Cells Using Spin Technology
By onTotal RNA can be efficiently purified from cells using spin technology, which involves binding RNA to a column, washing away contaminants, and eluting pure RNA for downstream applications.
Cytotoxicity Assay Protocol
By onThis is the protocol for cytotoxicity assay in cancer cell lines using flow cytometry.
v2 RNAscope
By onThis is a protocol for performing RNAscope® in situ hybridization analysis on fixed-frozen mouse brain tissue using the RNAscope® Multiplex Fluorescent v2 kit (Advanced Cell Diagnostics; ACD).
Descending Platform
By onBehavioral test to assess motor function in rodents using the descending inclined platform.
Rotarod (40 RPM and 2- RPM Docking)
By onMotor function behavioral test in mice using rotarod apparatus.
EEG Surgery- Telemetry implantation and recording
By onStandard Operating Procedure for EEG surgery including the preparation of EEG transponder (DSI), anesthesia and animal preparation, calibration, and recording.
Quantification of glutamate released from human induced pluripotent stem cells (iPSC) derived cortical neurons (CNs)
By onThis protocol describes the procedure to quantify glutamate released from induced pluripotent stem cells derived cortical neurons (iPSC-CNs) cultured in adherent monolayer.
Measuring dopamine release in human-derived iPSCs using High-Performance Liquid Chromatography (HPLC) coupled to Electrochemical Detection (ECD)
By onThis protocol allows for the detection of evoked or tonic dopamine release from human iPSC-derived dopamine neurons using High-Performance Liquid Chromatography (HPLC) coupled to electrochemical detection.
Electromyogram recordings for internal capsule stimulations in non-human primates
By onThis protocol outlines steps for performing EMG recordings to confirm implant location during internal capsule stimulation in monkeys. Supplementary to Liang et al. 2024 10.1016/j.jneumeth.2024.110133.
Western Blot in Mouse Brain Tissue for detecting pRab proteins
By onProtocol for detecting pRab proteins in mouse brain tissue.
Assessment of implant accuracy using high-resolution postmortem MRI
By onThis protocol describes the steps to use postmortem imaging to assess the accuracy of an implant by visualizing implant location with a small thermal ablation and comparing its coordinates to image-based pre-surgical planning in nonhuman primates.
Cross-modality frame alignment for surgical robot electrode insertion
By onThis protocol details step by step how to use stereotaxic frame features to align (or coregister) monkey head CT and MR images in a clinical ROSA ONE Brain robot in preparation for electrode insertion planning.
Ventral Midbrain Genomic PCR
By onProtocol outlines ventral midbrain genomic PCR for research purposes.
Immunofluorescent Imaging and Analysis
By onFollowing treatment with antibodies/genetic methods of introducing fluorescent tags.
Isothermal Titration Calorimetry of the Rubicon RH domain and Rab7
By on1:1 binding of Rubicon and Rab7.
