Metabolomics Preparation and Analysis
By onProtocol for metabolomic analysis from mouse fecal and cecal samples.
Metagenome Preparation and Analysis
By onProtocol for metagenomic analysis from mouse fecal pellets.
Whole Gut Transit Time, Fecal Water Content, and Fecal Output
By onProtocol for mouse gastrointestinal function assays (whole gut transit time, fecal water content, and fecal output)
Colonic Migrating Motor Complexes
By onProtocol for ex vivo assay of colonic migrating motor complexes (CMMCs) in mouse colons.
Y-Maze Protocol
By onThe Y maze assesses rodents' spatial working memory by observing their tendency to explore new arms over revisiting old ones. Rodents with intact memory will alternate between arms before revisiting.
SOP FOR MESOSCALE DISCOVERY ASSAYS
By onStandard Operating Procedure (SOP) for conducting mesoscale discovery assays, detailing the step-by-step process for accurate and efficient experimentation.
SOP for TriZOL RNA and Protein Extraction
By onA Standard Operating Procedure (SOP) for extracting RNA and protein using TriZOL, a common reagent for isolating nucleic acids and proteins from biological samples.
SOP for Flow cytometry after DSS-induced gut and brain injury
By onStandard Operating Procedure (SOP) for conducting flow cytometry analysis on samples from mice with DSS-induced gut and brain injuries.
SOP for LBP ELISA after DSS-induced injury
By onA standard operating procedure (SOP) for performing LBP ELISA after inducing injury with DSS.
Quantitative PCR, 384 well format
By on384-well format is used in quantitative PCR to increase throughput, allowing simultaneous analysis of multiple samples in a high-throughput manner.
SOP for BCA protein assay and western immunoblotting
By onSOP for BCA protein assay and Western immunoblotting includes detailed steps for protein quantification and detection using specific reagents, equipment, and protocols to ensure accurate results in biochemical research.
SOP for cDNA synthesis by RT (Reverse Transcription) Promega kit
By onSOP for cDNA synthesis by RT (Reverse Transcription) Promega kit.
N_terminal protein labeling
By onThis protocol details how to efficiently label a protein at the N-terminus using Clusterin protein as example.
Formation and isolation of Clu phospholipid particles
By onThis protocol details how to efficiently make in vitro and isolate Clu-phospholipid particles using purified Clusterin from HEK293E cells (dx.doi.org/10.17504/protocols.io.bvvkn64w) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC).
Very low-density lipoprotein receptor (VLDLR)-C-tag purification from HEK293E cells
By onThis protocol details how to purify recombinant Very low-density lipoprotein receptor (VLDLR)-C-tag protein from HEK293E cells.
Clusterin cellular uptake assay
By onThis protocol details how to efficiently monitor Clusterin and Clusterin/Substrate uptake in different cell types, like HEK293T, iNeurons and iMicroglia.
Amyloid beta (Aβ) aggregates N-terminal labeling
By onThis protocol details how to efficiently label protein aggregates at the N-terminal using Amyloid beta as example.
Solid phase binding assay – Clusterin binding to Very Low-Density Lipoprotein Receptor (VLDLR)
By onThis protocol details how to monitor Clusterin binding to the Very Low-Density Lipoprotein Receptor (VLDLR) by Enzyme-linked immunosorbent assays (ELISA) adapted from Leeb et al. (2014).
