Mutations in ATP13A2 gene can cause familial Parkinson's disease. Deleting ATP13A2 in adult mice leads to dopaminergic nerve terminal loss and neuronal degeneration, mimicking symptoms of ATP13A2-related neurodegenerative diseases.
This protocol outlines steps for performing ex vivo slice whole-cell patch-clamp electrophysiology experiments, including optogenetic stimulation introduced through viral injection or transgenic expression.
