Stereotaxic virus injection and fiber optic probe implantation
Output Details
Description
This protocol serves to prepare mice for in vivo optogenetic stimulation. Opsins will be expressed locally in the area where the injection is performed. In the control group, only the fluorescent protein YFP is expressed but not the opsin so that the neurons are not excited or inhibited when exposed to light. In this regard, if it is necessary to further restrict the expression of opsin and YFP protein (or YFP protein alone in controls) to a specific neuronal subpopulation, it is important to use a Cre line that is representative of the neural population of interest.
Identifier (DOI)
10.17504/protocols.io.yxmvmd92nv3p/v1
