Measuring alpha-synuclein concentration in hiPSCs using ELISA

By Iona Thomas-Wright and Richard Wade-Martins, PhD
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Output Details

Description
This protocol, carried out according to manufacturer’s instructions uses a classical ELISA experimental design with sensitive electrochemical detection to provide measure of 𝜶-synuclein concentration in the conditioned media of cultured iPSC-derived human cells.
Identifier (DOI)
10.17504/protocols.io.rm7vzxb65gx1/v1
Tags
  • 2D cultures
  • Alpha-synuclein
  • Cell culture
  • ELISA
  • Expression
  • hiPSCs (Human induced pluripotent stem cells)
Team
Team Cragg
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

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