GI Transit Assay
By onThis test is used to check for possible signs of constipation and alteration in gut transit.
Behavioral Testing – Open Field and Dyskinesia Scoring
By onThis protocol describes behavioral testing to assess motor deficits in mice. It includes open field locomotor testing and scoring of levodopa-induced dyskinesia.
HyDrop-RNA v1.0
By onStep-by-step protocol for performing HyDrop-RNA. The duration of each step assumes an experienced protocol user. For a first-time user, we recommend doubling the expected time for each step.
Investigation_of_mitophagy_in_Hippo_neurons
By onWe developed a method for assessing mitochondrial clearance in primary hippocampal neurons.
Halo assay to assess mitophagy
By onThis protocol describes how to assess mitophagy using Halo assay developed by Mizushima lab (DOI: 10.7554/eLife.78923).
Essential surgeries for the electrophysiological recording from a behaving non-human primate brain 1 (Head fixation post and recording chamber implantation)
By onProtocol for implanting head fixation post and recording chamber in non-human primates for electrophysiological recording.
Quantification of Extracellular 𝜶-synuclein Assay
By onThis protocol uses a classical ELISA experimental design with sensitive electrochemical detection to provide measure of 𝜶-synuclein concentration in the conditioned media of cultured iPSC-derived human cells.
Immunoprecipitation (IP)
By onThis protocol details about immunoprecipitation using anti-HA magnetic beads.
Transcardiac Perfusion of Mouse for Brain Tissue
By onTranscardiac perfusion is a method used for clearing blood and preserving the mouse brain for immunostaining.
LRRK2 microtubule sedimentation binding assay
By onAssay to determine LRRK2 protein binding to microtubules
In vitro phosphatase assay
By onThis protocol describes the procedure to perform in vitro phosphatase assay.
Collection of protocols of Team Deleidi used in the publication: “”LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson’s disease””
By onCollection of protocols of Team Deleidi used in the publication: ""LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson’s disease""
GUV assay
By onThis protocol describes the preparation of GUVs for the reconstitution of LC3 lipidation reaction through the steps of PI3P production by PI3KC3-C1, WIPI2 recruitment and LC3 lipidation with PE. Screen reader support enabled.
LRRK2RCKW Widefield fluorescence microtubule binding assay
By onThis assay uses TMR labeled LRRK2 or LRRK1 RCKW to measure binding to microtubules in vitro
Whole-cell Patch-Clamp Recordings from Striatal Cholinergic Interneurons in ex vivo Mouse Brain Slices
By onProtocol for patch-clamp recording of mCherry-labelled striatal ChIs from mouse brain slices with added step for labelling astrocytes using SR101.
RNA and protein extraction from bulk dissections
By onProtocol for RNA and protein extraction from bulk dissections.
Microsomal membrane isolation from cell culture
By onMicrosomal membrane isolation from cell culture
Flow cytometry-based measurement of trafficking of Golgi to the lysosome via autophagy
By onA protocol was developed to measure Golgi trafficking to lysosomes through autophagy in HEK293 cells using Keima-YIPF3/4 reporters with flow cytometry.
