mCherry-YIPF4 Immunoprecipitation V2
By onThe protocol outlines how to immunoprecipitate mCherry-YIPF4 for further analysis.
Immunoblotting of macrophages and microglia
By onThis protocol describes the preparation from cell lysate from cultured cells and immunoblotting procedure.
Mitochondrial complex activity assays
By onMitochondria complex activity assays measure the activity levels of the different complexes of the mitochondrial electron transport chain (ETC).
Midbrain viral injections for striatal fiber photometry in mice
By onThis protocol describes how to: - Inject AAV viruses into the midbrain (SNc: substantia nigra pars compacta, or VTA: ventral tegmental area) of mice - Train mice for head-fixed running on a cylindrical treadmill, and to receive rewards and air puffs while head-fixed - Implant head plates on mice for head-fixation during behavior (optional) While this protocol is focused on injections of AAV viruses into the midbrain, it can be easily modified to inject into other brain regions or other viruses.
Gene editing of YIPF4, CALCOCO1, and ATG7 in HeLa cells
By onThe text outlines the process of generating YIPF4, FIP200, and ATG7 knockout cell lines in HeLa cells through CRISPR-Cas9 technology.
Endogenous coimmunoprecipitation
By onProtocol used for immunoprecipitation of HSP60 and LONP1 in HEK cells to show the interaction with V5-Flag-tagged WT-GCase
Transferrin uptake assay to measure Clathrin-mediated endocytosis in HIPCS derived neurons
By onThis protocol is used to measure Transferrin uptake as a way to investigate Clathrin-mediated endocytosis in HIPCS derived neurons, or other cell types.
In Vivo Carbon Fiber Electrode Thread (CFET) Implantation and Testing Procedures
By onProcedures for implanting and testing CFET devices in rats are described.
Primary neuron culture for live imaging of axonal cargoes
By onThis protocol describes the preparation and culture of mouse primary cortical neurons for live-imaging experiments. Neurons were transfected 16-24 hours before imaging using Lipofectamine 2000.
Microscopy-based evaluation of mtKeima flux in hESC-derived Ctrl and FBXO7-/- iNeurons
By onProtocol for the microscopy-based evaluation of mtKeima flux in hESC-derived Ctrl and FBXO7-/- iNeurons PARK15/FBXO7 is dispensable for PINK1/Parkin mitophagy in iNeurons and HeLa cell systems: https://www.embopress.org/doi/full/10.15252/embr.202256399
Fast Scan Cyclic Voltammetry (FSCV) in Mouse Brain Slices
By onThis protocol describes the method of fast scan cyclic voltammetry (FSCV) for the application of measuring dopamine transients in mouse brain slices. Within the protocol are sections describing the manufacture of microelectrodes, preparation of acute brain slices, fiber calibration, and measuring stimulated dopamine release.
Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism – ASAP protocol collection
By onCollection of protocols of Deleidi Lab used in the publication: "Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism."
Biochemical detection of aggregated Tau
By onThis protocol is associated with the following preprint, published on February 19th 2022: The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds Itika Saha, Patricia Yuste-Checa, Miguel Da Silva Padilha, Qiang Guo, Roman Körner, Hauke Holthusen, Victoria A. Trinkaus, Irina Dudanova, Rubén Fernández-Busnadiego, Wolfgang Baumeister, David W. Sanders, Saurabh Gautam, Marc I. Diamond, F. Ulrich Hartl, Mark S. Hipp bioRxiv 2022.02.18.481043; doi: https://doi.org/10.1101/2022.02.18.481043
Microscopy-based bead protein-protein interaction assay
By onAn assay to study protein-protein interaction
Lentivirus production
By onThis protocol is associated with the following preprint, published on February 19, 2022: “The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds.”
Stereotaxic Injections
By onStereotaxic injections for Conserved and cell type-specific transcriptional responses to IFN-γ in the ventral midbrain.
