GUV assay
By onThis protocol describes the preparation of GUVs for the reconstitution of LC3 lipidation reaction through the steps of PI3P production by PI3KC3-C1, WIPI2 recruitment and LC3 lipidation with PE. Screen reader support enabled.
Collection of protocols of Team Deleidi used in the publication: “”LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson’s disease””
By onCollection of protocols of Team Deleidi used in the publication: ""LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson’s disease""
Whole-cell Patch-Clamp Recordings from Striatal Cholinergic Interneurons in ex vivo Mouse Brain Slices
By onProtocol for patch-clamp recording of mCherry-labelled striatal ChIs from mouse brain slices with added step for labelling astrocytes using SR101.
RNA and protein extraction from bulk dissections
By onProtocol for RNA and protein extraction from bulk dissections.
Microsomal membrane isolation from cell culture
By onMicrosomal membrane isolation from cell culture
Flow cytometry-based measurement of trafficking of Golgi to the lysosome via autophagy
By onA protocol was developed to measure Golgi trafficking to lysosomes through autophagy in HEK293 cells using Keima-YIPF3/4 reporters with flow cytometry.
MBP Pulldown Assay of ATG9A Truncations
By onProtocol describing MBP Pulldown Assay of ATG9A Truncations.
Immunoprecipitation of NAP1-GFP
By onThis protocol describes the procedure to perform immunoprecipitation of NAP1-GFP from HAP1 cells.
Proximity biotinylation of ATG8 proteins and selective autophagy receptors
By onAutophagy forms autophagosomes around cellular cargo for degradation in lysosomes. Proximity biotinylation with APEX2 fused to ATG8 proteins identifies proteins near autophagic cargo receptors during selective autophagy activation by nutrient stress
Analysis of glycosphingolipids from human plasma
By onThis is an updated protocol with the focus on achieving sensitive and reproducible quantitation of glycosphingolipids from human plasma samples
Immunohistochemistry of AAV-treated tissues
By onOutlines procedures to perform immunohistochemistry on tissue that had been treated with AAV.
Fluorescent Immunolabelling for endogenous ChAT in mice striatum
By onThis immunohistochemistry protocol has been designed and adjusted specifically for ChAT immunolabelling in the mice striatum.
Evaluation of mtKeima foci in induced neurons (iNeurons)
By onAssociated with preprint: https://www.biorxiv.org/content/10.1101/2022.11.02.514817v1
Electrophysiological recording from a chronic chamber-implanted non-human primate
By onThis protocol describes the preparation and insertion of extracellular recording electrodes into intracranial targets of a non-human primate implanted with a chronic recording chamber.
Mito-Keima assay to assess mitophagy
By onThis protocol details the procedure of mito-Keima assay to assess mitophagy.
Single-cell Whole Genome Amplification (scWGA) of human post-mortem brain samples isolated by Laser Capture Microdissection (LCM)
By onThis protocol uses Laser Capture Microdissection (LCM) technology on human post-mortem brain tissue slides in order to do low coverage single-cell whole genome sequencing to detect mega-base somatic Copy Number Variations (CNVs).
