LC3-lipidation-assay
By onProtocol for an in vitro LC3 lipidation assay using purified proteins and synthetic liposomes.
Western blot – alpha-synuclein
By onThis protocol describes how to detect alpha-synuclein in protein derived from STC-1 cells by western blot using DAB/peroxidase or ECL to visualize the bands.
Creating pooled CRISPR-Cas9 knock-outs in NIH-3T3 cells
By onTo validate a genome wide CRISPR screen, the authors select the top hits and create lentiviruses to validate the hits.
Challenging Beam Test
By onThe challenging beam test is used to measure motor performance, coordination, and balance.
Mouse Brain Tissue Collection and Analysis
By onThis protocol describes the dissection and collection of coronal sections of the striatum and midbrain from a mouse brain. The tissue can be used in a number of applications and here we describe two: the measurement catecholamine levels using high-performance liquid chromatography (aided by a neurochemistry core) and the distribution of the vesicular monoamine transporter (VMAT2) using a radioligand binding assay.
Halo-LC3B processing assay to assess autophagy
By onThis protocol details Halo-LC3B processing assay to assess autophagy.
Wire Hang Test
By onThis behavioral test is designed to assess early motor deficits associated with muscle and grip strength.
Reconstitution of LRRK2 membrane recruitment onto planar lipid bilayers
By onA method to monitor the recruitment of purified LRRK2 kinase onto planar lipid bilayers containing lipid-anchored Rab10 protein using Total Internal Reflection Fluorescence (TIRF) Microscopy.
Cell viability assessment
By onThis is the protocol for assessing cell viability using a MTS cell proliferation assay kit.
HeLa culture, transfection, and labeling of Halo-fusion proteins
By onProtocol for HeLa culture, transfection, and labelling of Halo-fusion proteins.
Surgical procedures for simultaneous optogenetic manipulation in the SNc and acute fiber photometry in the dorsal striatum
By onThis protocol describes the three surgical procedures required for simultaneous optogenetic stimulation in the Substantia Nigra pars compacta (SNc) and acute fiber photometry in the dorsal striatum.
Essential surgeries for the electrophysiological recording from a behaving non-human primate brain 2 (Craniotomy)
By onProtocol for performing a craniotomy in a recording chamber on a cranial implant in nonhuman primates.
Assay for Dual Rab GTPase binding to the LRRK2 Armadillo Domain
By onThe LRRK2 Armadillo domain contains multiple Rab GTPase binding sites. This assay shows that the sites can be occupied simultaneously.
Evaluating endocytic rate in cells
By onAssess endocytic rate in cells using tagged transferrin (Alexa647) and flow cytometry readout.
CRISPR-Cas9 screen in NIH-3T3 cells to identify modulators of LRRK2 function
By onThis protocol describes a pooled, CRISPR Cas9 screen to identify modulators of LRRK2 activity.
Flow cytometry-based measurement of mitophagic flux
By onAssociated with preprint: https://www.biorxiv.org/content/10.1101/2022.11.02.514817v1
LC3 Lipidation assay for ATG3 Mutants
By onProtocol describing the procedure to perform LC3 lipidation reaction assay on liposomes (SUVs, Small Unilamellar Vesicles).
Assay for PhosphoRab activation of LRRK2 Kinase
By onThis protocol includes a method to produce phosphoRab8A protein and remove as much contaminating MST3 as possible, to enable use of the phosphoRab to test subsequent activation of LRRK2 kinase.
