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  • Fast Scan Cyclic Voltammetry (FSCV) in Mouse Brain Slices

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    This protocol describes the method of fast scan cyclic voltammetry (FSCV) for the application of measuring dopamine transients in mouse brain slices. Within the protocol are sections describing the manufacture of microelectrodes, preparation of acute brain slices, fiber calibration, and measuring stimulated dopamine release.

  • GST pulldown assay

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    Protocol describing how to perform a GST pulldown assay.

  • Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism – ASAP protocol collection

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    Collection of protocols of Deleidi Lab used in the publication: "Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism."

  • Biochemical detection of aggregated Tau

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    This protocol is associated with the following preprint, published on February 19th 2022: The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds Itika Saha, Patricia Yuste-Checa, Miguel Da Silva Padilha, Qiang Guo, Roman Körner, Hauke Holthusen, Victoria A. Trinkaus, Irina Dudanova, Rubén Fernández-Busnadiego, Wolfgang Baumeister, David W. Sanders, Saurabh Gautam, Marc I. Diamond, F. Ulrich Hartl, Mark S. Hipp bioRxiv 2022.02.18.481043; doi: https://doi.org/10.1101/2022.02.18.481043

  • SDS-PAGE and western blot analysis

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    This protocol describes SDS-PAGE and western blot analysis.

  • GFP-ATG3 GUV Assay

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    LC3 lipidation reaction assay on Giant Unilamellar vesicles (GUVs).

  • Lentivirus production

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    This protocol is associated with the following preprint, published on February 19, 2022: “The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds.”

  • Microscopy-based bead protein-protein interaction assay

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    An assay to study protein-protein interaction

  • Intraperitoneal L-dopa injection in mice

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    Intraperitoneal L-dopa injection in mice.

  • Stereotaxic Injections

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    Stereotaxic injections for Conserved and cell type-specific transcriptional responses to IFN-γ in the ventral midbrain.

  • Glucose Tolerance Test

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    The glucose tolerance test is performed to determine how quickly the glucose is cleared from the blood. It is used to test for diabetes or insulin resistance.

  • Striatal Cannula Window Implantation

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    This protocol describes how to implant a cannula window over the dorsolateral striatum in mice to enable two-photon imaging of dopamine axons in the dorsal striatum.

  • Workflow for proteomic analysis of purified lysosomes in cells lacking GRN V.3

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    Associated with the following preprint (published September 30th 2021): Progranulin deficiency results in reduced bis(monoacylglycero)phosphate (BMP) levels and gangliosidosis Sebastian Boland, Sharan Swarup, Yohannes A. Ambaw, Ruth C. Richards, Alexander W. Fischer, Shubham Singh, Geetika Aggarwal, Salvatore Spina, Alissa L. Nana, Lea T. Grinberg, William W. Seeley, Michal A. Surma, Christian Klose, Joao A. Paulo, Andrew D. Nguyen, J. Wade Harper, Tobias C. Walther, Robert V. Farese Jr. bioRxiv 2021.09.30.461806; doi: https://doi.org/10.1101/2021.09.30.461806

  • In vitro kinase assay: phosphorylation of PI3Kc1 by TBK1and ULK1

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    Protocol describing in vitro kinase assay, in which PI3Kc is tested for phosphorylation by TBK1 and ULK1 complex either by western blotting (S29 on ATG14L subunit) or mass spectrometry analysis.

  • Brain processing, slicing and immunohistochemistry protocol

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    This protocol is a step by step procedure from collecting brain samples to immunohistochemical staining and mounting brain slices.

  • Mouse Ovariectomy

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    This protocol describes the steps to perform an ovariectomy in a mouse. This procedure is used to induce menopause.

  • Pole Test to assess motor coordination in parkinsonian mice

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    The pole test evaluates the motor coordination ability of a mouse by having them grasp a pole and then descend to its home cage.

  • HyDrop Bead Generation & PCR Barcoding v1.0

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    Protocol for producing dissolvable barcoded hydrogel beads used in HyDrop experiments.

  • Mitochondrial Antigen Presentation in RAW macrophages

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    This protocol details methods for 3-day Mitochondrial Antigen Presentation Assay in a murine macrophage cell line (RAW) that expresses a glycoprotein B (gB) of herpes simplex virus 1 (HSV1) targeted to the mitochondrial matrix (mito-gB). A gB-specific CD8+ T cell hybridoma recognizing the gB498–505peptide loaded on MHC class I molecules is also used to monitor antigen presentation through a beta-galactosidase assay kit.

  • Measurement of GLP-1 release in cell supernatant from Hutu-80 enteroendocrine cells via ELISA

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    Measurement of GLP-1 release in cell supernatant from Hutu-80 enteroendocrine cells via ELISA

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