Single-cell dissociation of brain organoids
By onThis protocol details single cell dissociation of brain organoids. This protocol is part of a Collection of protocols (dx.doi.org/10.17504/protocols.io.8epv593dng1b/v1) for the paper "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function" (https://doi.org/10.21203/rs.3.rs-1521848/v1)
Cell culture, transfection, immunocytochemistry, and imaging
By onThis protocol is to help with the maintenance, transfection, immunocytochemistry, and imaging of adherent mammalian cells
Expansion of mouse embryonic fibroblasts (MEFs) for hPSC cultures
By onThis protocol describes the expansion of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell (hPSC) culture.
iNeuron differentiation from human iPSCs
By onThe authors adapted a previously-described method for differentiating iPSCs stably expressing mNGN2 at a safe-harbor locus into human excitatory glutamatergic neurons.
HEK293 cell culture for co-immunoprecipitation experiments
By onProtocol describing how to perform HEK293 cell culture for the purpose of co-immunoprecipitation experiments.
Generation of stable cell lines using viral infection
By onProtocol for generation and precipitation of retrovirus, and infection of HeLa cells to generate stable cell lines.
Cell Culture
By onThis protocol describes routine cell culture maintenance as described in Adriaenssens & Nguyen et al., 2023 (BioRxiv)
Membrane and cytosol fractionation
By onThis protocol describes membrane and cytosol fractionation of cells expressing different DNAJC5 isoforms.
Mitochondria purification
By onThis protocol describes how to purify mitochondria from cell culture.
shRNA knockdown
By onThis protocol describes a standard procedure to generate a stable cell line for a DNAJC5 knockdown using shRNA.
Differentiation of SH-SY5Y cells
By onThis protocol describes a standard procedure to differentiate SH-SY5Y cells into dopaminergic neurons using retinoic acid
Immunoblots
By onThe steps of this protocol are similar for different sources of protein such as cell lysate, cytosol, or membrane samples, and EV samples.
Neuronal transdifferentiation from human primary adult fibroblasts
By onThis is a protocol for the direct conversion of human primary fibroblasts into neurons using a combination of transcription factor- and small molecule-based approach. The majority of converted neurons showing characteristics of cortical glutamatergic neurons.
Lentivirus preparation for neuronal transdifferentiation
By onThis is a protocol for the preparation of Lentiviruses.
Fabrication of fluid-walled dumbbells and generation of the human corticostriatal pathway
By onThis protocol described the generation of fluid-walled dumbbells for culturing of induced pluripotent stem cell-derived neurons with directional connectivity so as to recapitulate the human corticostriatal connectivity.
Automatic flow in fluid-walled dumbbells driven by Laplace pressure
By onThis protocol describes experiments performed to quantify pressure and volume variations inside fluid-walled dumbbells when a pressure difference is generated between the two chambers.
Generation of stable cell lines using retroviral system
By onThis protocol details the procedure for the generation of stable cell lines using retroviral system.
A human development-based protocol for the differentiation of human ESCs into midbrain dopaminergic neurons.
By onProtocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons.
Patient PBMC differentiation to macrophages
By onDifferentiation of patient peripheral blood mononuclear cells (PBMCs) into macrophages is a common method used in research to study immune responses and diseases.
