High-Yield Monocyte/Macrophage Differentiation from hiPSC
By onHere, a highly efficient method for differentiating monocytes/macrophages from hiPSC is described. The process utilizes commercially-available materials to derive CD34+ progenitor cells that are apically released from a hemogenic endothelium. Subsequently, the hemogenic endothelium gives rise to highly pure (>95%), CD34-CD14+ monocytes in 19-23 days and yields more monocytes by day 35 when compared to previous methods. These monocytes are further differentiated to macrophages after 7 days. The efficient workflow and increase in monocyte output boost feasibility for high throughput studies and enables clinical-scale iPSC-derived manufacturing processes.
Poly-ornithine/laminin substrate for neural cell culture V.2
By onProtocol to generate a sticky substrate for neurons.
hPSC Passaging and Propagation on laminin521 V.1
By onHighly efficient passaging and propagation of human PSC.
Maintenance & Differentiation: SHSY-5Y Neuroblastoma Cells
By onProtocol outlines SHSY-5Y neuroblastoma cell upkeep and differentiation process.
Primary glia isolation and culture protocol
By onProtocol isolates and maintains microglia, oligodendrocytes, and astrocytes from early postnatal primary mouse cortex.
Human Embryonic Kidney Cells (HEK-293)
By onProtocol for maintaining and culturing HEK-293 cells is outlined.
Maintenance & Differentiation: Embryonic Mouse Hippocampal Cells (CLU198)
By onProtocol details maintenance, differentiation, and harvesting of CLU198 mouse hippocampal cell line.
T cell purification and activation
By onThis protocol details the purification and activation of Mouse Naïve CD8+ T Cell from mouse spleen
ASO transfection of iPSC-derived cells
By onA protocol for transfecting iPSC-derived midbrain dopaminergic neurons with antisense oligonucleotides (ASOs).
Culturing 3T3 Cells for Validating GEARBOCS Constructs
By onProtocol for culturing Cas9 NIH/3T3 cells to validate GEARBOCS 2.0 and similar constructs for in vivo assays
Ex vivo culture of SPMs protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By onEx vivo culture of SPMs protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration"
Induced Cortical Neuron Differentiation – Part 3 – Day 7 Dissociation
By onProtocol for replating neurons after seven days of cortical transdifferentiation and prepare cells for longer-term culture.
Induced Cortical Neuron Differentiation – Part 2 – Day 0 Dissociation
By onProtocol to initiate differentiation of human iPS cells into cortical neurons by doxycycline induction of NGN2 transgene expression. Use iPSCs transfected with an NGN2-puroSNAP PiggyBac construct, with a blasticidin-resistance backbone.
Induced Cortical Neuron Differentiation – Part 1 – Overview
By onProtocol to differentiate human iPS cells into cortical neurons by doxycycline induction of NGN2 transgene expression. Use iPSCs transfected with an NGN2-puro-SNAP PiggyBac construct, with a blasticidin-resistance backbone.
Generation of preformed αS fibrils
By onThis protocol describes the generation of wild-type recombinant alpha-synuclein preformed fibrils (PFFs).
Stable integration of piggyBac plasmids into U2OS cells
By onGeneration of stably transfected U2OS human osteosarcoma cell line with piggyBac constructs.
Differentiation of WTC11 and KOLF2.1 iPSCs to dopaminergic neurons (forked)
By onThis protocol describes the differentiation of iPSCs (WTC11 and KOLF2.1) to dopaminergic neurons according to Bressan et al 2021.
Neuronal co-culture
By onThis protocol describes the co-culturing of iPSC-derived dopaminergic (DA) neurons and iPSC-derived medium spiny neurons (MSNs) in a microfluidic compartmentalization device.
