Maintenance & Differentiation: Embryonic Mouse Hippocampal Cells (CLU198)
By onProtocol details maintenance, differentiation, and harvesting of CLU198 mouse hippocampal cell line.
T cell purification and activation
By onThis protocol details the purification and activation of Mouse Naïve CD8+ T Cell from mouse spleen
ASO transfection of iPSC-derived cells
By onA protocol for transfecting iPSC-derived midbrain dopaminergic neurons with antisense oligonucleotides (ASOs).
Culturing 3T3 Cells for Validating GEARBOCS Constructs
By onProtocol for culturing Cas9 NIH/3T3 cells to validate GEARBOCS 2.0 and similar constructs for in vivo assays
Ex vivo culture of SPMs protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By onEx vivo culture of SPMs protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration"
Induced Cortical Neuron Differentiation – Part 3 – Day 7 Dissociation
By onProtocol for replating neurons after seven days of cortical transdifferentiation and prepare cells for longer-term culture.
Induced Cortical Neuron Differentiation – Part 2 – Day 0 Dissociation
By onProtocol to initiate differentiation of human iPS cells into cortical neurons by doxycycline induction of NGN2 transgene expression. Use iPSCs transfected with an NGN2-puroSNAP PiggyBac construct, with a blasticidin-resistance backbone.
Induced Cortical Neuron Differentiation – Part 1 – Overview
By onProtocol to differentiate human iPS cells into cortical neurons by doxycycline induction of NGN2 transgene expression. Use iPSCs transfected with an NGN2-puro-SNAP PiggyBac construct, with a blasticidin-resistance backbone.
Generation of preformed αS fibrils
By onThis protocol describes the generation of wild-type recombinant alpha-synuclein preformed fibrils (PFFs).
Stable integration of piggyBac plasmids into U2OS cells
By onGeneration of stably transfected U2OS human osteosarcoma cell line with piggyBac constructs.
Differentiation of WTC11 and KOLF2.1 iPSCs to dopaminergic neurons (forked)
By onThis protocol describes the differentiation of iPSCs (WTC11 and KOLF2.1) to dopaminergic neurons according to Bressan et al 2021.
Neuronal co-culture
By onThis protocol describes the co-culturing of iPSC-derived dopaminergic (DA) neurons and iPSC-derived medium spiny neurons (MSNs) in a microfluidic compartmentalization device.
Cell culture, transfection, immunocytochemistry, and imaging (iPSC, iNeuorn, DA neuron, RPE cells)
By onThis protocol describes the maintenance, transfection, immunocytochemistry, and imaging of RPE1 and also transfection, immunocytochemistry, and imaging of iPSCs, i3 Neurons and DA neurons.
Generation of knockout iPSCs using CRISPR-Cas9 genome editing
By onThis protocol describes the genetic modification of induced pluripotent cells (iPSCs) using CRISPR-Cas9, including synthesis of gRNA plasmids, transfection, selection of clones, and sequencing of genomic DNA to confirm knockout generation. The steps
Primary hippocampal and cortical neuronal culture and transfection
By onThis protocol presents an established method to isolate and culture hippocampal and cortical neurons from rat and mouse brains along with a method to sparsely transfect these neurons.
Astrocyte generation
By onA protocol for generating astrocytes from cortical neural progenitor cells using a commercial astrocyte media (AM) is described.
