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Primary hippocampal and cortical neuronal culture and transfection
Output Details
Description
Neurons consist some of the most complex cells. The culture of primary neurons from murine brains has advanced the understanding of neuronal function at the cellular and molecular level. This protocol presents an established method to isolate and culture hippocampal and cortical neurons from rat and mouse brains along with a method to sparsely transfect these neurons.
Identifier (DOI)
10.17504/protocols.io.ewov1qxr2gr2/v1