Standard operating procedure for the isolation of genetically engineered hPSCs clones in a high-throughput way
By onThis collection describes a standard procedure for isolating single human pluripotent stem cell (hPSC) clones in a high-throughput way.
Midbrain-like Organoids generation from hiPSCs
By onProtocol for generating midbrain organoids from human iPSCs.
Passaging of feeder-free hPSCs
By onThis protocol describes the process of passaging human pluripotent stem cells (hPSCs) for feeder-free culturing of hPSCs using Accutase or ReLeSF.
Preparing feeder-free hPSCs for nucleofection
By onThis protocol describes the standard procedure preparing feeder-free human pluripotent stem cells (hPSCs) for nucleofection.
Thawing of feeder-free hPSCs
By onThis protocol describes the procedure of thawing feeder-free human pluripotent stem cells (hPSCs) using mTeSR-plus or StemFlex.
Freezing of feeder-free hPSCs
By onThis protocol describes the process of freezing feeder-free human pluripotent stem cells (hPSCs) using Accutase or ReLeSR
Culture and transfection of iPSC-derived neurons for live-imaging of axonal cargoes
By onThe authors plate, culture, and transfect human iPSC-derived excitatory glutamatergic neurons for the purpose of observing transport of axonal cargoes under spinning disk confocal microscopy.
Maintenance and inactivation of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell culture
By onThis collection describes the maintenance and inactivation of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell (hPSC) culture.
Nucleofection of hPSCs
By onThis protocol describes the standard procedure for the delivery of plasmids, mRNA, or ribonucleoprotein (RNP) into human pluripotent stem cells (hPSCs) using nucleofection.
Thawing of mouse embryonic fibroblasts (MEFs) for hPSC cultures
By onThis protocol describes the thawing of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell (hPSC) culture.
Determination of edits in CRISPR-edited cell lines by sequencing
By onThis protocol details the procedure of determination of edits in CRISPR-edited cell lines by sequencing.
Harvesting and irradiation of mouse embryonic fibroblasts (MEFs) for hPSC cultures
By onThis protocol describes the process of harvesting and irradiating mouse embryonic fibroblasts (MEFs) to use as feeder cells for human pluripotent stem cell (hPSC) culture.
Single-cell dissociation of brain organoids
By onThis protocol details single cell dissociation of brain organoids. This protocol is part of a Collection of protocols (dx.doi.org/10.17504/protocols.io.8epv593dng1b/v1) for the paper "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function" (https://doi.org/10.21203/rs.3.rs-1521848/v1)
Expansion of mouse embryonic fibroblasts (MEFs) for hPSC cultures
By onThis protocol describes the expansion of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell (hPSC) culture.
Cell culture, transfection, immunocytochemistry, and imaging
By onThis protocol is to help with the maintenance, transfection, immunocytochemistry, and imaging of adherent mammalian cells
HEK293 cell culture for co-immunoprecipitation experiments
By onProtocol describing how to perform HEK293 cell culture for the purpose of co-immunoprecipitation experiments.
iNeuron differentiation from human iPSCs
By onThe authors adapted a previously-described method for differentiating iPSCs stably expressing mNGN2 at a safe-harbor locus into human excitatory glutamatergic neurons.
Generation of stable cell lines using viral infection
By onProtocol for generation and precipitation of retrovirus, and infection of HeLa cells to generate stable cell lines.
Cell Culture
By onThis protocol describes routine cell culture maintenance as described in Adriaenssens & Nguyen et al., 2023 (BioRxiv)
Membrane and cytosol fractionation
By onThis protocol describes membrane and cytosol fractionation of cells expressing different DNAJC5 isoforms.
