Gene editing of YIPF4 in hESCs V3
By onThis protocol describes the creation of YIPF4 knockout cell lines in H9 hESC cells using CRISPR-Cas9. Associated with preprint: https://doi.org/10.1101/2022.12.06.519342
CRISPR tagging of the EEA1 gene in H9 ES cells for Endo-IP
By onProtocol for tagging EEA1 gene in human H9 ES cells with a 3X flag using CAS9 and oligonucleotide template is described.
Preparation of mouse embryonic fibroblast (MEF) feeder plates for hPSC cultures
By onThis protocol describes the preparation of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell (hPSC) culture.
Yeast cells live fluorescence imaging
By onProtocol for sample preparation and live fluorescence imaging of yeast cells.
iPSC differentiation into Microglia
By onThis protocol describes iPSC differentiation into microglia.
Hippocampal Neuronal Culture
By onThis protocol describes the procedure for hippocampal neuronal cultures from new - born mouse pups.
Astrocyte extraction from brain organoids V.2
By onProtocol for astrocyte extraction from brain organoids.
Standard operating procedure for the isolation of genetically engineered hPSCs clones in a high-throughput way
By onThis collection describes a standard procedure for isolating single human pluripotent stem cell (hPSC) clones in a high-throughput way.
Bone Marrow Derived Macrophage (BMDM) differentiation and maintenance
By onThis protocol describes the isolation of bone marrow derived macrophages.
Midbrain-like Organoids generation from hiPSCs
By onProtocol for generating midbrain organoids from human iPSCs.
Passaging of feeder-free hPSCs
By onThis protocol describes the process of passaging human pluripotent stem cells (hPSCs) for feeder-free culturing of hPSCs using Accutase or ReLeSF.
Preparing feeder-free hPSCs for nucleofection
By onThis protocol describes the standard procedure preparing feeder-free human pluripotent stem cells (hPSCs) for nucleofection.
Thawing of feeder-free hPSCs
By onThis protocol describes the procedure of thawing feeder-free human pluripotent stem cells (hPSCs) using mTeSR-plus or StemFlex.
Culture and transfection of iPSC-derived neurons for live-imaging of axonal cargoes
By onThe authors plate, culture, and transfect human iPSC-derived excitatory glutamatergic neurons for the purpose of observing transport of axonal cargoes under spinning disk confocal microscopy.
Freezing of feeder-free hPSCs
By onThis protocol describes the process of freezing feeder-free human pluripotent stem cells (hPSCs) using Accutase or ReLeSR
Maintenance and inactivation of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell culture
By onThis collection describes the maintenance and inactivation of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell (hPSC) culture.
Nucleofection of hPSCs
By onThis protocol describes the standard procedure for the delivery of plasmids, mRNA, or ribonucleoprotein (RNP) into human pluripotent stem cells (hPSCs) using nucleofection.
Thawing of mouse embryonic fibroblasts (MEFs) for hPSC cultures
By onThis protocol describes the thawing of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell (hPSC) culture.
Determination of edits in CRISPR-edited cell lines by sequencing
By onThis protocol details the procedure of determination of edits in CRISPR-edited cell lines by sequencing.
Harvesting and irradiation of mouse embryonic fibroblasts (MEFs) for hPSC cultures
By onThis protocol describes the process of harvesting and irradiating mouse embryonic fibroblasts (MEFs) to use as feeder cells for human pluripotent stem cell (hPSC) culture.
