Harvesting and culturing of peritoneal macrophages and ex-vivo stimulation
By onThe process involves harvesting and culturing peritoneal macrophages followed by ex-vivo stimulation with IFNy for research purposes.
Protocol for α-synuclein (αSyn) proximity ligation assay (PLA) for detecting α-synuclein oligomers in rodents
By onProtocol for α-synuclein (αSyn) proximity ligation assay (PLA) for detecting α-synuclein oligomers in rodents.
Induction of aggregation in alpha-synuclein-expressing cells by treatment with preformed fibrils (PFFs)
By onThis protocol details how to efficiently produce alpha-synuclein aggregates in cells by templating the misfolding of intracellular alpha-synuclein through treatment with preformed fibrils of alpha-synuclein (PFFs).
Immunofluorescence on human brain FFPE sections to identify neuromelanin in A6, A9, and A10
By onA protocol to scan brain neuromelanin with reduced impact of the chemicals on their original color and intensity. The immunofluorescence staining protocol is designed to identify human A6, A9, and A10 cell clusters and alpha-synuclein pathology.
Preparing 10X Genomics CytAssist FFPE Sections
By onDesigning and properly mounting FFPE sections on large-window CytAssist slides is important for downstream analysis and data quality. This protocol describes the key steps for this preparation.
Immunophenotyping of immune cells by high dimensional flow cytometry
By onProtocol to assess cellular phenotypes by multiparametric flow cytometry panels containing markers to identify cell types and activation states.
Immunophenotyping of the peripheral blood immune cells by flow cytometry
By onProtocol for immunophenotyping peripheral blood immune cells.
Optical densitometry of tyrosine hydroxylase fibers
By onProtocol for quantifying TH+ fibers in rat striatum and substantia nigra pars reticulata (SNpr)
Stereology-mediated cell count using StereoInvestigator
By onProtocol for cell counting using StereoInvestigator software.
STC-1 Passaging and PD Associated Microbe Stimulation Protocol
By onProtocol for passaging STC-1 cells involves stimulating with microbes to induce proliferation. This process can be used to study cell behavior and responses.
SOP for Immune cells isolation mouse adult brain with CD45 beads
By onThe SOP outlines the procedure for isolating immune cells from adult mouse brain using CD45 beads for subsequent analysis or experiments.
Passaging and Seeding Mouse Embryonic Fibroblasts (MEFs) for Experiments
By onProtocol for passaging and seeding mouse embryonic fibroblasts (MEFs) outlined.
Stable cell line generation via retrovirus
By onProduction of pseudotyped virus an subsequent transduction.
Embryonic/Postnatal Mouse Neuron Culture Protocol
By onThis protocol details the primary neuron culture procedures from embryonic and postnatal mice.
Preparation of human iPSC-derived cortical neuronal progenitors for transplantation into the rodent brain
By onThis protocol describes how we prepare human iPSC-derived cells, that have been differentiated into cortical neuronal progenitors, for transplantation into the brain of immunocompromised athymic mice. Neuronal progenitors mature within the mouse brain and are used to study the pathogenesis of Parkinson’s disease.
Generation of stable LysoTag expressing cell lines and LysoTag immunoprecipitation of lysosomes
By onProtocol for generating LysoTag (TMEM192-3xHA) cells and immunoprecipitation of lysosomes for subsequent -omics analyses.
Differentiation of human cortical neurons (CNs) from induced pluripotent stem cells (iPSCs)
By onThis protocol described the production of human cortical neurons from induced pluripotent stem cells. The differentiation protocol of cortical neurons is adapted and modified from Shi, Y. et al., 2012 (pharmacologically directed) and Zhang, Y. et al., 2013 (transcription factor directed), as previously described by Hedegaard et.al., 2020. These neurons exhibited molecular and functional hallmarks of cortical neurons.
Culturing iPSC cells in Essential 8 medium
By onCulturing of iPSC lines in essential 8 medium, thawing and passaging techniques.
dopaminergic neurons from iPSC
By onThis protocol describes our method for the differentiation of human floor plate neural progenitor cells into human midbrain-dopaminergic neurons. This protocol has been developed using a combination of several published protocols.
