Immunophenotyping of the peripheral blood immune cells by flow cytometry
By onProtocol for immunophenotyping peripheral blood immune cells.
Optical densitometry of tyrosine hydroxylase fibers
By onProtocol for quantifying TH+ fibers in rat striatum and substantia nigra pars reticulata (SNpr)
Stereology-mediated cell count using StereoInvestigator
By onProtocol for cell counting using StereoInvestigator software.
STC-1 Passaging and PD Associated Microbe Stimulation Protocol
By onProtocol for passaging STC-1 cells involves stimulating with microbes to induce proliferation. This process can be used to study cell behavior and responses.
SOP for Immune cells isolation mouse adult brain with CD45 beads
By onThe SOP outlines the procedure for isolating immune cells from adult mouse brain using CD45 beads for subsequent analysis or experiments.
Passaging and Seeding Mouse Embryonic Fibroblasts (MEFs) for Experiments
By onProtocol for passaging and seeding mouse embryonic fibroblasts (MEFs) outlined.
Stable cell line generation via retrovirus
By onProduction of pseudotyped virus an subsequent transduction.
Embryonic/Postnatal Mouse Neuron Culture Protocol
By onThis protocol details the primary neuron culture procedures from embryonic and postnatal mice.
Preparation of human iPSC-derived cortical neuronal progenitors for transplantation into the rodent brain
By onThis protocol describes how we prepare human iPSC-derived cells, that have been differentiated into cortical neuronal progenitors, for transplantation into the brain of immunocompromised athymic mice. Neuronal progenitors mature within the mouse brain and are used to study the pathogenesis of Parkinson’s disease.
Generation of stable LysoTag expressing cell lines and LysoTag immunoprecipitation of lysosomes
By onProtocol for generating LysoTag (TMEM192-3xHA) cells and immunoprecipitation of lysosomes for subsequent -omics analyses.
Differentiation of human cortical neurons (CNs) from induced pluripotent stem cells (iPSCs)
By onThis protocol described the production of human cortical neurons from induced pluripotent stem cells. The differentiation protocol of cortical neurons is adapted and modified from Shi, Y. et al., 2012 (pharmacologically directed) and Zhang, Y. et al., 2013 (transcription factor directed), as previously described by Hedegaard et.al., 2020. These neurons exhibited molecular and functional hallmarks of cortical neurons.
Culturing iPSC cells in Essential 8 medium
By onCulturing of iPSC lines in essential 8 medium, thawing and passaging techniques.
dopaminergic neurons from iPSC
By onThis protocol describes our method for the differentiation of human floor plate neural progenitor cells into human midbrain-dopaminergic neurons. This protocol has been developed using a combination of several published protocols.
High-Yield Monocyte/Macrophage Differentiation from hiPSC
By onHere, a highly efficient method for differentiating monocytes/macrophages from hiPSC is described. The process utilizes commercially-available materials to derive CD34+ progenitor cells that are apically released from a hemogenic endothelium. Subsequently, the hemogenic endothelium gives rise to highly pure (>95%), CD34-CD14+ monocytes in 19-23 days and yields more monocytes by day 35 when compared to previous methods. These monocytes are further differentiated to macrophages after 7 days. The efficient workflow and increase in monocyte output boost feasibility for high throughput studies and enables clinical-scale iPSC-derived manufacturing processes.
Poly-ornithine/laminin substrate for neural cell culture V.2
By onProtocol to generate a sticky substrate for neurons.
hPSC Passaging and Propagation on laminin521 V.1
By onHighly efficient passaging and propagation of human PSC.
Maintenance & Differentiation: SHSY-5Y Neuroblastoma Cells
By onProtocol outlines SHSY-5Y neuroblastoma cell upkeep and differentiation process.
Primary glia isolation and culture protocol
By onProtocol isolates and maintains microglia, oligodendrocytes, and astrocytes from early postnatal primary mouse cortex.
Human Embryonic Kidney Cells (HEK-293)
By onProtocol for maintaining and culturing HEK-293 cells is outlined.
