Fabrication of fluid-walled dumbbells and generation of the human corticostriatal pathway

Description

This protocol described the generation of fluid-walled dumbbells for culturing of induced pluripotent stem cell-derived neurons with directional connectivity so as to recapitulate the human corticostriatal connectivity. The fluid-walled microfluidic technology was first reported by Walsh et al., 2017 and subsequently shown to be adaptable for cell culture.

Identifier (DOI)

10.17504/protocols.io.kqdg39zb1g25/v1

Tags

2D cultures
Axons
Cell culture
hiPSCs (Human induced pluripotent stem cells)
Microfluidic devices

Team

Team Cragg

Program

Collaborative Research Network

Theme

Circuitry and Brain-Body Interactions

Meet the Authors

Federico Nebuloni

External Collaborator
Quyen Do

Key Personnel: Team Cragg,

University of Oxford
Richard Wade-Martins, PhD

Co-PI (Core Leadership): Team Cragg,

University of Oxford

Fabrication of fluid-walled dumbbells and generation of the human corticostriatal pathway

Output Details

Meet the Authors

Federico Nebuloni

Quyen Do

Richard Wade-Martins, PhD

Related Research

Datasets and Key Resources Table used in Do, Quyen B. et al. (2023) “Early striatal hyperexcitability in an in vitro human striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation”

Early deficits in an in vitro striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation

STC-1 cell culture