Standard Operating Procedure: Mouse Spinal Cord Injection Surgery
By onSOP outlines procedures for mouse spinal cord injection surgery.
Rotarod
By onRotarod protocol optimized for mice. This test is made to assess motor coordination and balance. The results should show lower latency to fall numbers for mice with motor impairments.
Gut-PFF Surgery
By onMethod for injection of alpha-synuclein preformed fibrils in the gut in a mice.
Protocol for neuromelanin accumulation drives endogenous synucleinopathy in non-human primates
By onCollection of protocols used in this publication
AAV Craniotomy
By onThis protocol is for injection of AAV into a mouse brain using a stereotactic frame and Hamilton syringe.
6-OHDA lesion in medial forebrain bundle
By onLesion of the medial forebrain bundle using 6-OHDA to induce parkinsonian-like deficits in mice.
Transgenic mouse colony maintenance
By onMethods for the generation and maintenance of transgenic tyrosinase mice
In vivo microdialysis for striatal DA release
By onTo assess local effects of nomifensine (DAT and NET inhibitor) on striatal DA release in microdialysis experiments
Seizures – like behavior assay for D. melanogaster
By onThis protocol allows to assess the susceptibility of flies to seizure-like behavior based on mechanically hyper-stimulating sensory inputs and assessing seizure-like activity.
Genetic interaction analysis using electroretinograms (ERGs) in D. melanogaster
By onThis protocols describes the recording of electroretinograms (ERGs) and calculation of genetic interactions of fly mutants for a transheterozygous screen.
Treatment of D. melanogaster with small molecules in fly food
By onThis protocol describes the preparation of fly food with small molecules to treat flies during their aging. It can be adapted for any treatments. Details for small molecules used can be found in the "Materials" section
Behavior monitoring of D. melanogaster using ethoscopes
By onThis protocol describes the practical steps to perform behavioral monitoring using ethoscopes. The cleaning and preparation of glass tubes as well as loading the flies into ethoscopes is outlined.
Development and characterization of a non-human primate model of diseeminated synucleinopathy
By onA viral vector was used in NHP to spread a mutated gene, inducing synucleinopathy in brain areas connected to the putamen. After 4 weeks, animals were studied to quantify affected neurons and their distribution.
Rotarod test protocol used in”LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By onRotarod test protocol used in"LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration"
Animal housing and ethical concerns protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By onAnimal housing and ethical concerns protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration"
Experimental design protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By onExperimental design protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration"
