Optogenetic modulation of dopaminergic neurons
By onThis protocol describes an optogenetic activation of neuromelanine-laden dopaminergic neurons in the SNc-VTA.
Chemogenetic modulation of catecholaminergic neurons
By onThis protocol outlines a method for modulating noradrenergic and dopaminergic neuronal activity in the LC and SNc-VTA, respectively, using Cre-dependent hM3D and hM4D expression. It details stereotactic injection techniques for these chemogenetic tools and the preparation of the J60 agonist for intraperitoneal delivery. Developed for longitudinal studies spanning up to four weeks, the protocol aims to assess neuromelanin's activity-dependent accumulation through daily agonist administration.
Elevated plus maze test for mice
By onThe elevated plus maze is a classical test to screen anxiety-like behaviors in laboratory animals.
Open field test for mice
By onThe open field test is a classical test for the quantification of locomotor activity and anxiety-like behaviors in laboratory animals. This protocol is optimized for mice, and it includes the automatic analysis of their movement by Biobserve Viewer.
Parallel Beam test for mice
By onThe parallel beam test protocol is designed to evaluate the motor coordination of mice. This protocol is designed to assess motor skills, particularly in neurological diseases such as Parkinson's disease.
Light-dark box test for mice
By onThis protocol describes the setup and execution of the light-dark box test for mice, a behavioral experiment used to assess anxiety-related responses. The protocol involves preparing a light-dark box in a controlled environment, utilizing specific lighting conditions and camera settings for optimal observation and recording.
Rotarod-Test for Mice
By onThe Rotarod Test Protocol for Mice assesses their motor coordination and balance. Mice are trained to walk on a rotating rod in a dimly lit environment and then tested for their ability to maintain balance as the rod accelerates from 4 to 40 RPM. The test, repeated thrice with intervals, measures the latency to fall and speed at the fall. This protocol is vital to study neurological disease such as Parkinson disease.
Locomotion test for mice
By onThe locomotion test is an open field test in darkness, so it does not aim to measure anxiety, only locomotor activity.
Standard Operating Procedure: Mouse Spinal Cord Injection Surgery
By onSOP outlines procedures for mouse spinal cord injection surgery.
Rotarod
By onRotarod protocol optimized for mice. This test is made to assess motor coordination and balance. The results should show lower latency to fall numbers for mice with motor impairments.
Gut-PFF Surgery
By onMethod for injection of alpha-synuclein preformed fibrils in the gut in a mice.
Protocol for neuromelanin accumulation drives endogenous synucleinopathy in non-human primates
By onCollection of protocols used in this publication
6-OHDA lesion in medial forebrain bundle
By onLesion of the medial forebrain bundle using 6-OHDA to induce parkinsonian-like deficits in mice.
AAV Craniotomy
By onThis protocol is for injection of AAV into a mouse brain using a stereotactic frame and Hamilton syringe.
Transgenic mouse colony maintenance
By onMethods for the generation and maintenance of transgenic tyrosinase mice
In vivo microdialysis for striatal DA release
By onTo assess local effects of nomifensine (DAT and NET inhibitor) on striatal DA release in microdialysis experiments