Standard Operating Procedure (SOP) for Combined ICA and Systemic Administration of MPTP: The Overlesioned (Bilateral Asymmetric) Non-human Primate Model
By onThis standard operating procedure (SOP) describes safe methods for the use of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in non-human primates (adult macaque monkeys).
Generation of A549 PPM1H BromoTag CRISPR/CAS9 knock-in cell line
By onThis protocol details the generation of A549 PPM1H BromoTag CRISPR/CAS9 knock-in cell line.
Sleep-Wake Recording in Dan lab
By onSurgery and Setups for Recording 24-Hour Natural Sleep/Wake Cycles in Mice.
Sleep-Wake Recording in the Turek Laboratory
By onSurgery and recording protocol for measuring sleep-wake behavior in mice for Turek Laboratory.
Localised axotomy of human Cortical Neurons (CNs) from induced pluripotent stem cells (iPSCs)
By onThis protocol describes the process followed to perform localised axotomy of iPSC-derived human cortical axons cultured within fluid-walled dumbbells in 6 cm TCT-treated Petri dishes.
Viral-mediated short-hairpin RNA knockdown
By onStereotaxic injection of viral vectors for gene knockdown experiments: The AAV vectors for knocking down the gene of interest were custom-made with Vector Biolabs using AAV-GFP-U6 vector for AAV1 packaging (Cat no, 7040, Vector Biolabs).
Preparation and Administration of Systemic 4-AP
By onProcedure for preparation and systemic administration of 4-AP used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
CRISPR nuclease (CRISPRn) genome editing
By onProcedure for CRISPR nuclease (CRISPRn) genome editing used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
CRISPR knock-in (endogenous tagging)
By onProcedure for CRISPR knock-in (endogenous tagging) used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
CRISPR knock-in validation
By onProcedure for CRISPR knock-in validation used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
Quantification of p62-positive inclusions
By onQuantification of p62-positive inclusions in the rodent brain
Magnetic resonance imaging (MRI) of mouse brain
By onMagnetic resonance imaging (MRI) of mouse brain.
