Protocol selection for Saurat et al. CSC 2024 V.2
By onProtocol collection for the study "Genome-wide CRISPR screen reveals neddylation's role in neuronal aging and AD neurodegeneration" by Saurat et al. in Cell Stem Cell 2024. Contains 20 protocols. Full manuscript: DOI: 10.1016/j.stem.2024.06.001.
Protocol selection for Kim et al. Cell 2024 V.2
By onKim et al. in Cell 2024 published "TNF-NF-kB-p53 axis restricts in vivo survival of hPSC-derived dopamine neurons" with 13 protocols. Full text at (https://doi.org/10.1016/j.cell.2024.05.030).
Standard Operating Procedure (SOP) for systemic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in non-human primates
By onThis protocol details standard operating procedure (SOP) for systemic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in non-human primates.
Mouse Behavior – Open Field and T-Maze
By onThis protocol describes two behavioral tasks for mice. The first is the Open Field Test, which is used to asses motor behavior, and the second is the T-Maze, which is used to assess spatial learning.
In Vivo Electrophysiology (Mouse)
By onThis protocol describes the in vivo electrophysiology method for recording neuronal activity in mice.
Accelerated Ovarian Failure AOF mouse model: a model of transitional menopause
By onThe novel model of AOF successfully replicates the human peri- and post-menopause stages, including erratic estrous cycles and hormone fluctuations, followed by undetectable estrogen levels.
Collection of protocols for paper: “Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function”
By onThis is a collection of protocols used in a recent preprint by the Deleidi Lab, Team Schapira. You can access pre-print at https://doi.org/10.21203/rs.3.rs-1521848/v1
Gavage – food supplementation for stalled weight gain in mice
By onFeeding protocol for Ndufs2fl/fl mice,
Mouse Stereotaxic Surgery
By onThis protocol describes the steps for performing stereotaxic surgery in mice. It is applicable to intracranial injections (e.g. virus, drug) and placement of implants (e.g. optical fibers, electrode arrays) into targeted regions of mouse brains.
Protocols for “Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice”
By onThis is a collection of protocols for the manuscript "Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice"
Deep Brain Stimulation (DBS) Implant
By onThis protocols describing the steps for making implants used for deep brain stimulation (DBS) in rodents.
Optogenetic Manipulation (Mouse)
By onThis protocol describes the steps for in vivo ontogenetic manipulation in mice, including assembly of fiber-ferrules, surgical implantation of fibers, and testing procedure.
Fiber Photometry (Mouse)
By onThis protocol describes the procedure for fiber photometry in awake behaving mice. It includes details on the surgical implantation of fibers.
Ex Vivo Electrophysiology
By onThis protocol describes preparation of brain slices, setup of electrophysiology rig, and solutions for collecting whole cell and cell attached recordings.
AAV injection in the nodose ganglia of mouse
By onThe gut-brain axis links the visceral organs to the medulla oblongata via the vagus nerve. Accessing to the afferent vagal pathway is important to dissect the role of cell populations in the bidirectional communication between brain and body. The jugular-nodose ganglion (JNG) complex contains the cell bodies of many heterogenous neural subpopulations responsible for sensing the physiological and pathological conditions of the thoracic and abdominal organs. However, the study of these ganglia is challenging in small animals due to size and location. Hence, in this protocol we describe a practical surgical approach to the vagal trunk, and the JNG complex in mice.
