Genetic interaction analysis using electroretinograms (ERGs) in D. melanogaster
By onThis protocols describes the recording of electroretinograms (ERGs) and calculation of genetic interactions of fly mutants for a transheterozygous screen.
Seizures – like behavior assay for D. melanogaster
By onThis protocol allows to assess the susceptibility of flies to seizure-like behavior based on mechanically hyper-stimulating sensory inputs and assessing seizure-like activity.
Behavior monitoring of D. melanogaster using ethoscopes
By onThis protocol describes the practical steps to perform behavioral monitoring using ethoscopes. The cleaning and preparation of glass tubes as well as loading the flies into ethoscopes is outlined.
Treatment of D. melanogaster with small molecules in fly food
By onThis protocol describes the preparation of fly food with small molecules to treat flies during their aging. It can be adapted for any treatments. Details for small molecules used can be found in the "Materials" section
Development and characterization of a non-human primate model of diseeminated synucleinopathy
By onA viral vector was used in NHP to spread a mutated gene, inducing synucleinopathy in brain areas connected to the putamen. After 4 weeks, animals were studied to quantify affected neurons and their distribution.
Rotarod test protocol used in”LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By onRotarod test protocol used in"LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration"
Animal housing and ethical concerns protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By onAnimal housing and ethical concerns protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration"
Experimental design protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By onExperimental design protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration"
PFF seeding in iPSC-derived cortical neurons
By onHow to seed intracellular inclusions via exogenous addition of short-length pre-formed aSyn fibrils.
Stereotaxic Injections- Mouse
By onThis protocol details mouse stereotaxic injections for any purpose, but in this case is primarily used for the injection of misfolded proteins.
Stereotaxic injections into mouse brain and ex-vivo electrophysiology
By onProtocol outlines injection of α-Synuclin PFF and monomer into mouse brain, followed by preparation of acute slices from mice for whole-cell patch clamp recordings.
Collection of protocols for “PINK1 deficiency rewires early immune responses in a mouse model of Parkinson’s disease triggered by intestinal infection”
By onThis a collection of protocols for "PINK1 deficiency rewires early immune responses in a mouse model of Parkinson’s disease triggered by intestinal infection".
Mouse EEG implantation and monitoring
By onProtocol for EEG implantation surgery and EEG data collection for mouse models
Unilateral 6-hydroxydopamine lesion mouse model of Parkinson’s disease
By onTo generate a mouse model of Parkinson's disease, unilateral injection of 6-OHDA(6-hydroxydopamine) into the medial forebrain bundle (MFB) was performed. Three to four weeks later, a drug-free cylinder test was used to assess the extent of lesion.
