Stereotaxic Injections- Mouse
By onThis protocol details mouse stereotaxic injections for any purpose, but in this case is primarily used for the injection of misfolded proteins.
Stereotaxic injections into mouse brain and ex-vivo electrophysiology
By onProtocol outlines injection of α-Synuclin PFF and monomer into mouse brain, followed by preparation of acute slices from mice for whole-cell patch clamp recordings.
Collection of protocols for “PINK1 deficiency rewires early immune responses in a mouse model of Parkinson’s disease triggered by intestinal infection”
By onThis a collection of protocols for "PINK1 deficiency rewires early immune responses in a mouse model of Parkinson’s disease triggered by intestinal infection".
Mouse EEG implantation and monitoring
By onProtocol for EEG implantation surgery and EEG data collection for mouse models
Unilateral 6-hydroxydopamine lesion mouse model of Parkinson’s disease
By onTo generate a mouse model of Parkinson's disease, unilateral injection of 6-OHDA(6-hydroxydopamine) into the medial forebrain bundle (MFB) was performed. Three to four weeks later, a drug-free cylinder test was used to assess the extent of lesion.
Chronic Cranial Window and Micro-Optical Probe Implantation in Mice
By onMonitoring neuronal structure and activity in behaving animals helps understand movement control. Surgical procedures like chronic cranial window and micro-optical probe implantation allow optical monitoring of neural circuits.
Tamoxifen Administration in Transgenic Mice-Ding Lab
By onThis protocol describes the procedure for inducing CreER-mediated recombination at loxP sites in transgenic mice through the administration of tamoxifen or 4-hydroxytamoxifen (4-OHT).
Costa Lab Nanoject Virus Injections
By onThis is the standard stereotaxic/Nanoject injection mouse surgery protocol for the Costa lab at Columbia University.
Stereotaxic intracranial virus injection in mice
By onThis protocol outlines steps for conducting stereotaxic brain injections of virus in mice in the Ding lab.
Stereotaxic Optic Fiber Implantation in Mice
By onThis protocol describes implantation of one or multiple optic fiber cannulae in mouse brains used for optical recording brain activity using fiber photometry or for optogenetic manipulation of neuronal activity in mice performing behavioral tasks.
6-OHDA mouse model of Parkinson’s disease
By onProtocol for creating a Parkinson's disease mouse model using 6-OHDA involves injecting different doses into the brain to induce varying levels of dopaminergic damage.
Levodopa treatment for low-dose 6-OHDA-treated mice
By onThis protocol describes a one-week levodopa treatment for mice partially depleted of dopamine.
Mouse Stereotaxic Surgery-Arber Lab
By onThis protocol describes the steps for performing stereotaxic surgery in mice. It is applicable to intracranial injections (e.g. virus, drug) and placement of implants (e.g. optical fibers, electrode arrays) into targeted regions of mouse brains.
Optimized Stereotactic Injection Protocol for Targeting the Locus Coeruleus with Minimal Neurotoxicity
By onRefined stereotactic injection protocol targeting the Locus Coeruleus minimizes neurotoxicity.
Stereotaxic virus injection in the SNpc of rats
By onProtocol for stereotaxic virus injection in the substantia nigra pars compacta of rats
Stereotactic injection of viral vectors
By onHere we describe stereotactic injection of AAV into ventral tegmental area (VTA) of mice.
Performing surgeries, Image processing and Analysis on Animals
By onThis protocol details the experimental procedures on animals that includes the surgery, two-photon imaging, cued lever-pushing task and analysis includes movement behavior analysis, and PCA.
