Viral-mediated short-hairpin RNA knockdown

Stereotaxic injection of viral vectors for gene knockdown experiments : The AAV vectors for knocking down the gene of interest were custom-made with Vector Biolabs using AAV-GFP-U6 vector for AAV1 packaging (Cat no, 7040, Vector Biolabs). They were then unilaterally injected into the striatum of mice as follows