Disrupted sleep has a profound adverse impact on lives of Parkinson’s Disease (PD) patients and their caregivers. Sleep disturbances are exceedingly common in PD, with substantial heterogeneity in type, timing, and severity. Among the most…
This protocol describes stereotaxic delivery of 6-hydroxydopamine (6-OHDA) to generate either a mild medial forebrain bundle (MFB) lesion model in 3- to 5-month-old wild-type C57BL/6J mice.
The protocol describes in detail how to induce GCase loss of function in mice using CBE treatment. Mice exhibit severe motor symptoms accompanied by neuroinflammation after four weeks of daily CBE injections using this protocol.
