pCAG-mcherry- WIPI2dK88E-cs- TEV -STREP
By onPlasmid for mammalian expression of human WIPI2d K88E with N-terminal mCherry and C-terminal Strep.
x4 GBA Plasmids
By onThe below plasmids are deposited and available via Addgene:https://www.addgene.org/Anthony_Schapira/ . These have been used in publication: 10.1093/hmg/ddac233 188580 WT GBA pcDNA3.1 GBA (Homo sapiens) 188581 E326K GBA pcDNA3.1 GBA (Homo sapiens) 188582 L444P GBA pcDNA3.1 GBA (Homo sapiens) 188583 N370S GBA pcDNA3.1 GBA (Homo sapiens)
Lentivirus plasmid: pLV[Exp]-U6>KAT8_P1_Seq1-hPGK>mApple
By onPlasmid: Plasmid vector encoding a sgRNA sequence which targets the human KAT8 promoter for CRISPRi, under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV backbone.
pCAG-MBP-ATG9-D830A/E831A
By onPlasmid for expression of human ATG9-D830A/E831A mutant in mammalian cells
pCAG- WIPI2dR125E- cs-TEV -STREP
By onPlasmid: Mammalian expression of human WIPI2d R125E with C-terminal Strep
Plasmid: pLV[Exp]-EF1A>V5/hKAT8[NM_032188.3]:IRES:Puro
By onPlasmid: Human KAT8 gene with N-terminal V5 tag cloned into the pLV plasmid backbone under a EF1a promoter and including IRES puromycin selection cassette by Vectorbuilder.
pCAG-OSF-ATG13 (2-197)-W50D
By onPlasmid for expression of human ATG13 HORMA W50D mutant in mammalian cells.
cDNA clones for expression of GolgiTag (TMEM115) in mammalian cells
By onPlasmids for expression of TMEM115 (GolgiTag) in human and mouse cells.
His-ATG3-K62D/K64D
By onPlasmid: ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K62D/K64D-stop.
pCAG-OSF-ATG13 (2-197)-Y152D
By onPlasmid for expression of human ATG13 HORMA Y152D mutant in mammalian cells.
