pCMV-pOTC-GFP
By onPlasmid: Expression of GFP fused with pOTC, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase.
pCAG-TSF-ATG13 (450-517)-ULK1 (836-1050)
By onPlasmid for mammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complex.
pFastBac_Dual_GST-TEV-mCherry-TBK1
By onPlasmid: Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631. Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675).
PGK EGFP-LC3B
By onPlasmid for mammalian expression of fluorescently-tagged LC3B, marker for autophagic vesicles.
cDNA clone for bacterial expression of human PPM1H D288A
By onPlasmid for bacterial expression of human PPM1H (D288A); contains TEV protease site for removal of 6His tag.
pCAG-MBP-Foldon-ATG9 (723-839)
By onPlasmid for expression of ATG9 C-terminal tail trimer in mammalian cells.
Lentivirus plasmid: pLV[Exp]-U6>PINK1_P1_Seq3-hPGK>mApple
By onPlasmid: Plasmid vector encoding a sgRNA sequence which targets the human PINK1 promoter for CRISPRi, under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV backbone.
pLenti HsATP13A3 D498N
By onTransfer plasmid for lenti viral vector production, Expresses D498N mutant of Homo sapiens ATP13A3