Protocol Archive

This protocol describes how to measure CD3 density in the substantia nigra and cerebral peduncle using haematoxylin and DAB-stained brain sections.

Procedures for mouse hippocampal cultures, lentivirus production, vGLUT1-pHluorin imaging and analysis used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."

This protocol describes how to measure CD8 density in the substantia nigra and cerebral peduncle using haematoxylin and DAB-stained brain sections.

Protocol for pHluorin assays, analysis, and fluorescence microscopy as performed in "Synapsin E-domain is essential for α-synuclein function." (https://doi.org/10.7554/eLife.89687.1)

Procedure for preparation and systemic administration of 4-AP used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."

Protocol for immunoprecipitation and Western blotting as performed in "Synapsin E-domain is essential for α-synuclein function." (https://doi.org/10.7554/eLife.89687.1)

Procedures for immunofluorescence and object-based colocalization analysis used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."

This protocol describes how to analyze AT8 tau pathology in human brain tissue (FFPE sections with IHC).

Protocol for GST fusion protein production as performed in "Synapsin E-domain is essential for α-synuclein function." (https://doi.org/10.7554/eLife.89687.1)

Procedure for proximity ligation assay used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."

Protocol for protein pull-down assays as performed in "Synapsin E-domain is essential for α-synuclein function." (https://doi.org/10.7554/eLife.89687.1)

Procedure for immunohistochemistry used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."

Procedure for electron microscopy (EM) and quantification of inter-vesicular distances used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."

Procedure for CRISPR knock-in (endogenous tagging) used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."

Procedure for CRISPR nuclease (CRISPRn) genome editing used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."

Procedure for CRISPR knock-in validation used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."

Procedure for preparing and Neuro2A lysates for biochemical assays and evaluation used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."

Procedure for preparing enriched synaptic vesicle fractions used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."

Procedure for in vitro dephosphorylation used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."

Procedure for in vitro phosphorylation used in "Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function."