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Making Ca2+ – selective microelectrodes

By Jeffrey Stedehouder, Dorly Verdier, Arlette Kolta and Stephanie Cragg, PhD
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Output Details

Description
The following protocol describes how to make Ca2+ sensitive electrodes to measure extracellular Ca2+ concentrations at high spatiotemporal resolution in acute ex vivo brain slices. Glass micropipettes can be precisely (µm precision) located at/near a particular brain region/cell type of interest to measure rapid changes in Ca2+ concentrations.
Identifier (DOI)
10.17504/protocols.io.yxmvmeeqog3p/v1
Tags
  • Calcium
  • Equipment
  • Ex Vivo
  • Mouse
Team
Team Cragg
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

  • User avatar fallback logo

    Jeffrey Stedehouder

    External Collaborator

  • User avatar fallback logo

    Dorly Verdier

    External Collaborator

  • User avatar fallback logo

    Arlette Kolta

    External Collaborator

  • Stephanie Cragg, PhD

    Lead PI (Core Leadership): Team Cragg, 

    University of Oxford

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Aligning Science Across Parkinson's
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