Endogenous tagging of the YIPF4 gene with mNEON Green and imaging of these cells by microscopy

Description

This protocol describes a method to create cells in which the YIPF4 gene has been endogenously tagged with mNEON fluorescent protein. CRISPR-based gene editing was used to introduce mNEON Green into the N-terminus of YIPF4 in WT and FIP200-/- HEK293T cells. We also describe a protocol for imaging of mNEON-YIPF4 in Golgi using the Golgi marker GOLGB1.

Identifier (DOI)

10.17504/protocols.io.5jyl8pj9dg2w/v1

Tags

Fluorescence imaging
Microscopy

Team

Team Harper

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

J. Wade Harper

Lead PI (Core Leadership): Team Harper,

Harvard University
Sharan Swarup, PhD

Key Personnel: Team Harper,

Harvard University
Kelsey Hickey, PhD

Key Personnel: Team Harper,

Harvard University

Endogenous tagging of the YIPF4 gene with mNEON Green and imaging of these cells by microscopy

Output Details

Meet the Authors

J. Wade Harper

Sharan Swarup, PhD

Kelsey Hickey, PhD

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