Stereotaxic injections of viral vectors and chronic optical fiber implants in mouse brains

Research Community
Research Community

ASAP supports three programs and serves as a member of multiple consortia to advance research for the benefit of the global Parkinson’s disease community.
Explore
Highlights
Highlights

ASAP shares the impact that our initiative, network, and supported programs have had on providing new insights into Parkinson’s disease. Discover news, interviews, awards, and annual impact.
Explore
Open Science
Open Science

ASAP is dedicated to facilitating a research environment where meaningful collaboration, research-enabling resources, and data sharing provides the answers we need to understand, diagnose, and treat Parkinson’s disease. Discover our open science philosophy.
Explore
Tools & Resources
Tools & Resources

ASAP is committed to developing and openly sharing research outputs, including data, code, protocols, and key lab materials, to help the scientific community build upon existing work.
Explore

Description

This protocol outlines the surgical procedures for stereotaxic viral injections and chronic optical fiber implantation in the mouse striatum for monitoring acetylcholine, dopamine, and glutamate dynamics during Pavlovian learning and extinction using genetically encoded sensors, as well as for suppressing acetylcholine release with tetanus toxin.

Tags

Fiber photometry
Injections
Mouse
Other

Discovery Team

Team Cragg

Program

Collaborative Research Network

Theme

Circuitry and Brain-Body Interactions

Meet the Authors

Safa Bouabid

External Collaborator
Mark Howe, PhD

Co-PI (Core Leadership): Team Cragg,

Boston University

Stereotaxic injections of viral vectors and chronic optical fiber implants in mouse brains

Output Details

Meet the Authors

Safa Bouabid

Mark Howe, PhD

Related Research

FibPho: Analysis Protocol

Fiber Photometry Protocol

Reward perseveration is shaped by GABAA-mediated dopamine pauses: Fiber Photometry Data