ATG9A-vesicle isolation protocol_
By onHap1 cells were previously CRISPR engineered in our lab to contain ATG9A endogenously tagged on the C-terminus with mEGFP-3C-FLAG.
Confocal microscopy of DFP-treated cells
By onThis protocol details the confocal microscopy of DFP-Treated cells after fixation and staining.
MS sample prep Quantitative whole cell proteomics
By onDescribes sample preparation procedure for Quantitative Whole Cell Proteome Analysis by Mass Spectrometry
Purification of GST-FAM134C
By onThis protocol details the purification of GST-FAM134C and its analysis.
Co-immunoprecipitation ER-phagy receptors
By onThis protocol details the co-immunoprecipitation of ER-phagy receptors.
BNIP3-GST (E44A L47A D49A A50K Q51A) (ΔWIPI2)
By onExpression of recombinant protein for purification
BNIP3-GFPpFastBac-Dual_BNIP3(1-158aa)-EGFP- TEV-GST
By onExpression of recombinant protein for purification
BCL2L13-GST (I224A/L227A/W276A/I279A/I307A/V310 A) (ΔLIR1+3+4)
By onExpression of recombinant protein for purification
BCL2L13-GST (I224A/L227A/W276A/I279A) (ΔLIR1+3)
By onExpression of recombinant protein for purification
