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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Protocol
Proteome preparation and analysis
Protocol for proteomic analysis from mouse cecal samples.
Protocol
Mouse sample collection for metabolomics studies
Protocol used in the Mazmanian lab for collecting brain and gut tissues and plasma from mouse for metabolomics.
Protocol
Metabolomics preparation and analysis
Protocol for metabolomic analysis from mouse fecal and cecal samples.
Protocol
Tissue preparation, immunohistochemistry, imaging, and quantification
Protocol for neuronal imaging.
Protocol
3′ mRNA-sequencing
Protocol for 3′ mRNA sequencing of mouse gastrointestinal tract tissues.
Protocol
Whole gut transit time, fecal water content, and fecal output
Protocol for mouse gastrointestinal function assays (whole gut transit time, fecal water content, and fecal output).
Protocol
Colonic migrating motor complexes
Protocol for ex vivo assay of colonic migrating motor complexes (CMMCs) in mouse colons.
Protocol
Virus production and administration
Protocol for AAV production and administration to mice.
Protocol
Neural activation of the GI tract
Protocol for neuronal activation of the enteric nervous system.
Protocol
Quantitative targeted metabolomics for ASO mouse model using Biocrates Q500 platform
Protocol for using the Biocrates MxP-Q500 kit (BIOCRATES Life Science AG, Innsbruck, Austria), a commercially-available targeted metabolomics platform, to measure 634 metabolites across 26 biochemical classes from different tissues of the mouse.
Dataset
Data and code for “α-Synuclein Overexpression and the Microbiome Shape the Gut and Brain Metabolome in Mice”
Metabolomic data and analysis code associated with the article, “α-Synuclein Overexpression and the Microbiome Shape the Gut and Brain Metabolome in Mice.”
Protocol
GCaMP6f fluorescence in ex vivo intestinal preparation
Protocol for GCaMP6f imaging in ex vivo mouse intestinal tissue.
Article
α-Synuclein overexpression and the microbiome shape the gut and brain metabolome in mice
Pathological forms of α-synuclein contribute to Parkinson’s disease (PD). Most cases of PD are believed to arise from gene-environment interactions. Microbiome composition is altered in PD, and gut bacteria are causal to symptoms and pathology in animals. Here, the authors explore how the microbiome may impact PD-associated genetic risks.
Dataset
Fecal metagenomic sequencing data for PD patients and controls from Rush University Medical Center
Fecal metagenomic sequencing data associated with Boktor et al. (2023). This dataset includes samples from the Rush University Medical Center cohort.