ASAP CRN Cloud Release Notes – Version 2.0.0
By onVersion: 2.0.0 Release Date: December 11, 2024 This release notes document provides a concise overview of the updates and enhancements introduced in Version 2.0.0 of the CRN Cloud platform.
Extracellular space diffusion modelling identifies distinct functional advantages of archetypical glutamatergic and GABAergic synapse geometries
By onWe modelled extracellular diffusion in super-resolved images of live brain tissue neuropil. We found that extracellular space geometry shapes local diffusion and this has functional implications for signaling arising from synaptic spill-over.
ASAP CRN Cloud Release Notes – Version 1.0.0
By onVersion: 1.0.0 Release Date: June 25, 2024 This release notes document provides a concise overview of the updates and enhancements introduced in Version 1.0.0 of the CRN Cloud platform.
ASAP CRN Cloud Release Notes – Version 1.0.0-beta
By onVersion: 1.0.0-beta Release Date: March 6, 2024 This release notes document provides a concise overview of the updates and enhancements introduced in Version 1.0.0-beta of the CRN Cloud platform.
ASAP CRN Cloud Release Notes – Version 0.0.1
By onVersion: 0.0.1 Release Date: November 10, 2023 This release notes document provides a concise overview of Version 0.0.1 of the CRN Cloud platform.
Dephosphorylation of phosphorylated Rab GTPases by PPM phosphatases
By onProtocol measures phosphoRab GTPase dephosphorylation with PPM phosphatases using purified phosphoRab GTPases. Substrates generated by phosphorylating Rab GTPases with MST3 kinase are included.
Flowcytometry analysis of lysosomal pulldown with anti-TMEM192 magnetic beads from PBMCs
By onMethod described for staining intact lysosomes with lysotracker for flow cytometry. Analysis provides quantitative indication of organelle enrichment in immunoprecipitation, serving as quality control.
Sample Preparation for Proteomic Analysis of Isolated Mitochondria and Whole-Cell Extracts
By onProtocol outlines precise sample prep for mass spectrometry-based proteomics, emphasizing robust lysis, S-trap column protein capture, and optimized digestion for high-quality data on proteomic changes and post-translational modifications.
Mito-IP: Immunopurification of Mitochondria from MitoTag Cultured Cells
By onA novel method called 'Mito-IP' enables rapid and pure mitochondrial isolation in 10 minutes, supporting various analyses. This technique is being extended to isolate other organelles like lysosomes, Golgi, and peroxisomes using epitope tags.
GCase assay – Resorufin β-D-glucopyranoside
By onMethod presented for assessing lysosomal enzyme GBA1 activity using Resorufin substrate in low pH conditions to minimize non-lysosomal activity. Validated in GBA1 KO cells, compatible with various lysates and adjustable for different GBA1 levels.
Mouse brain immunohistochemistry – colorimetric analysis
By onProtocol for colorimetric analysis of mouse brain immunohistochemistry is described.
A CellProfiler computational pipeline to quantify localization of PPM1H on mitochondria
By onCellProfiler software pipeline quantifies PPM1H localization on fragmented mitochondria by hypotonic swelling, highlighting membrane contacts. Wild-type MEFs expressing PPM1H-mApple and GFP-Mito are imaged after treatment with oligomycin/antimycin.
p-S65-Ub sandwich ELISA
By onAssay measures Ubiquitin phosphorylation at Serine-65 in vivo using ELISA. Method previously detailed by Watzlawik et al. in their publication.
PINK1 WT/LRRK2 WT, PINK1 WT/LRRK2 RC, PINK1 KO/LRRK2 WT, PINK1 KO/LRRK2 RC
By onMice with LRRK2 R1441C and PINK1 KO mutations were crossed to create double mutant lines: LRRK2 WT/PINK1 WT, LRRK2 RC/PINK1 WT, LRRK2 WT/PINK1 KO, and LRRK2 RC/PINK1 KO.
LRRK2 R1441C/PINK1 Double mutant mouse embryonic fibroblasts
By onLRRK2 R1441C/PINK1 double mutant mouse embryonic fibroblasts were studied alongside homozygous mutant and littermatched wild-type cells.
Primary data1: Pathogenic LRRK2 mutations cause loss of primary cilia and Neurturin in striatal parvalbumin interneurons
By onPathogenic LRRK2 mutations lead to loss of primary cilia and Neurturin in striatal parvalbumin interneurons, as indicated in the primary data for the manuscript.
Primary data2: Pathogenic LRRK2 mutations cause loss of primary cilia and Neurturin in striatal parvalbumin interneurons
By on*LRRK2* mutations lead to loss of primary cilia and Neurturin in striatal parvalbumin interneurons, as found in the manuscript.
Isolation of brain eGFP-cholinergic interneurons by dissociation and flow cytometry
By onProtocol isolates GFP-expressing cholinergic interneurons from mouse dorsal striatum tissue using a method by Lynette Foo (2013).
Part1 Raw data for: Lysosomal glucocerebrosidase is needed for ciliary Hedgehog signaling: A convergent pathway contributing to Parkinson’s disease
By onRaw data for Figures 1-5 in a study on the role of lysosomal glucocerebrosidase in ciliary Hedgehog signaling, linking to Parkinson's disease.
Part2 Raw data for: Lysosomal glucocerebrosidase is needed for ciliary Hedgehog signaling: A convergent pathway contributing to Parkinson’s disease
By onThe raw data for Figure 6 in the manuscript supports the role of lysosomal glucocerebrosidase in ciliary Hedgehog signaling, linking it to Parkinson's disease.
