KOLF2.1 iPSCs AAVS1-NGN2
By Emma Sherrell oniPSCs from a British Caucasian male, modified with NEUROG2 using CRISPR/Cas9 in AAVS1 locus. Contains mutations in ARID2 (p.Pro197Hisfs*12) and COL3A1 (p.Gly1176Ser). Derived from skin fibroblasts of a 55-59-year-old male.
pACEBac1-POMP-PSMG1-PSMG2-PSMG3-PSMG4
By Emma Sherrell onExpression plasmid for human proteasome subunits.
pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4-PSMA5-PSMA6-PSMA7
By Emma Sherrell onExpression plasmid for human proteasome subunits.
pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-PSMB5-PSMB6-PSMB7-TEV-2xSTII
By Emma Sherrell onExpression plasmid for human proteasome subunits.
pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII-PSMB5-PSMB6-PSMB7
By Emma Sherrell onExpression plasmid for human proteasome subunits.
α-Synuclein aggregation decreases cortico-amygdala connectivity and impairs social behavior in mice
By Emma Sherrell onThe study suggests that early circuit modulation could be an effective approach to alleviate symptoms associated with α-Syn pathology, necessitating studies of functional consequences of α-Syn aggregation.
Multifiber array fabrication and calibration
By Emma Sherrell onThis protocol describes a multi-fiber photometry approach to track dopamine release in mice with high spatial and temporal resolution in multiple locations in the brain simultaneously.
Targeted optogenetic stimulations
By Emma Sherrell onThis protocol includes the steps for targeted optogenetic manipulation using a new micro-fiber array approach that measures and optogenetically manipulates local dynamics across multiple targeted locations simultaneously in behaving mice.
Multi-color fiber array imaging
By Emma Sherrell onThis protocol includes the steps for fiber array imaging setup and acquisition using a new micro-fiber array approach that measures and optogenetically manipulates local dynamics across multiple targeted locations simultaneously in behaving mice.
Stereotaxic viral injections and array implantation
By Emma Sherrell onThis protocol includes the stereotaxic viral injections and array implantation steps using a micro-fiber array approach that measures and optogenetically manipulates local dynamics across multiple targeted locations simultaneously in behaving mice.
Micro-CT scanning and fiber localization
By Emma Sherrell onThis protocol includes the micro-CT scanning and fiber localization steps for a micro-fiber array approach that measures and optogenetically manipulates local dynamics across multiple targeted locations simultaneously in behaving mice.
Immunohistology in mouse brain sections after whole-brain micro-CT scanning
By Emma Sherrell onThis protocol includes immunohistology steps performed in mouse brain sections to confirm viral expression and stain for neurons after whole-brain micro-CT scanning and contrast agent soaking procedure.
Freely moving imaging
By Emma Sherrell onThis protocol includes the steps for imaging in freely moving mice using a new micro-fiber array approach that measures and optogenetically manipulates local dynamics in multiple sites.
Head-fixed behavior
By Emma Sherrell onThis protocol includes the steps for imaging in head-fixed mice using a new micro-fiber array approach that measures and optogenetically manipulates local dynamics in multiple sites.
Preprocessing pipeline to establish and extract data from ROIs from multifiber photometry movies
By Emma Sherrell onPipeline and code are available for imaging data preprocessing using a micro-fiber array approach to measure and manipulate local dynamics in mice, allowing the study of cell-specific signals in 3D volumes.
Pipeline and MATLAB code for micro-CT-based fiber localization
By Emma Sherrell onPipeline and MATLAB code for a micro-CT-based method for precise fiber localization and atlas alignment after performing micro-fiber photometry in behaving mice.
Custom G-code used to jet-print microfluid-walled dumbbells
By Emma Sherrell onAll original G-code used to jet-print the microfluid-walled dumbbells described in Nebuloni, F. et al. (2024) study.
ATG3 research outputs_discontinued
By Emma Sherrell onThis Excel file lists all ATG3 discontinued research outputs as they were deemed to be "not effective."
README guide for code
By Emma Sherrell onThe purpose of this document is to provide guidance on how to write README files for code.
Mitochondrial antigen presentation (MitAP) to primary 2C CD8 T (proliferation and suppression) assay
By Emma Sherrell onThis protocol details the mitochondrial antigen presentation (MitAP) to primary 2C CD8 T (proliferation and suppression) assay in vivo
