L1-silenced iPSC line – Control (HS1_L1HS-CRISPRi_LacZ )
By onHuman iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
L1-silenced iPSC line (HS1_L1HS-CRISPRi_g2 )
By onHuman iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
L1-silenced iPSC line (HS2_L1HS-CRISPRi_LacZ)
By onHuman iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
L1-silenced iPSC line (HS2_L1HS-CRISPRi_g1)
By onHuman iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
A single Citrobacter rodentium infection in Pink1 knockout and wild type mice leads to regional blood-brain-barrier perturbation and glial activation without dopamine neuron axon terminal loss
By onWe tested whether a gut infection causes blood-brain barrier permeability and brain inflammation. Results showed increased BBB permeability in specific brain regions at 26 days while tight-junction and dopamine related proteins remained unchanged
Lrrk2 G2019S mutation incites increased cell-intrinsic neutrophil effector functions and intestinal inflammation in a model of infectious colitis
By onUsing single cell RNA sequencing, we demonstrate that LRRK2 G2019S is associated with an increased neutrophil presence in the colonic lamina propria during infection, Th17 skewing, upregulated Il17a, and greater colonic pathology.
LRRK2, lysosome damage, and Parkinson’s disease
By onRecent research has uncovered how LRRK2 kinase activity is regulated by recruitment to endolysosomal membranes, involving interactions with small GTPases and GABARAP. This sheds light on its role in lysosomal damage and Parkinson's disease.
all protocols related to ‘Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and Parkinsonian motor deficits’
By onall protocols related to 'Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and Parkinsonian motor deficits
LRRK2G2019S acts as a dominant interfering mutant in the context of iron overload
By onUsing a model macrophage cell line we showed that LRRK2-G2019S mutation affects iron homeostasis by dysregulating iron-related proteins and blocking ferritinophagy in iron overload conditions, suggesting a role in iron dysregulation in PD.
FSCV dopamine, spike, and LFP data used for validating chronic electrode implanted in aseptic semi-sealed chamber
By onFSCV dopamine, spike, and LFP data used for validating chronic electrode implanted in aseptic semi-sealed chamber in Choi et al. 2025 JNeurosciMethods.
Robust analytical methods for bis(monoacylglycero)phosphate profiling in health and disease
By onBMP is a crucial phospholipid in lysosomes, impacting metabolic balance and linked to neurodegenerative diseases. LC-MS methods are detailed for accurate BMP analysis, utilizing structural differences and a new CLN5 function for profiling.
Choice Reaction Time Reaching Task for Non-human Primate
By onThis protocol describes the behavioral task used with both healthy and MPTP-induced hemiparkinsonian non-human primates in the Turner Lab at the University of Pittsburgh, as well as the training procedure for introducing the task to naïve NHPs.
MatLab Code and Source Data for Analyzing Reach-related Single Unit Activity in the Internal Segment of the Globus Pallidus in Parkinsonian Macaque
By onSource data stored in a non-proprietary format, all MATLAB code, along with histology data prepared for the publication of our study on movement-related activity in the internal globus pallidus of parkinsonian macaques (Kase et al. 2025).
AutoMorphoTrack: An Automated Python Package for Organelle Morphology, Motility, and Colocalization Analysis in Live-Cell Imaging
By onAutoMorphoTrack is a Python package for analyzing organelle dynamics in live-cell imaging data. It automates detection, classification, tracking, and colocalization of mitochondria and lysosomes, enabling reproducible and quantitative analysis.
ASAP Research Round-Up | Q2 2025
In this second edition of the ASAP Research Round-Up, ASAP shares advancements in Q2 2025 across the ASAP portfolio that fill critical knowledge gaps, promote rapid dissemination of scientific insights, expand resource accessibility, and support the next generation of Parkinson’s researchers.
Needed ASAP: The Partnership Rewiring Neurodegeneration Research
Inside Precision Medicine reports on the new ASAP, Allen Institute, and Michael J. Fox Foundation's partnership to unite Collaborative Research Network (CRN) Cloud data from 3 million human cells across 9 brain regions from individuals with Parkinson’s into the Allen Brain Cell (ABC) Atlas visualization tool, which already contains 6.4 million human cells.
Expanded Research Tool to Crack the Code on Parkinson’s, the Fastest-Growing Neurodegenerative Disease
ASAP and Allen Institute announce a new collaboration that creates a common language for visualizing data from Parkinson’s and Alzheimer’s research
Collaboration Expands Research Tool to Help Crack the Code on Parkinson’s and Other Neurodegenerative Diseases
In this blog, ASAP and Allen Institute answer questions about our new collaboration that helps researchers unlock insights into Parkinson’s and other neurodegenerative diseases.
ASAP Collaborative Research Network (CRN) Impact Report
From 2020 to 2024, the ASAP CRN supported 35 multidisciplinary, international teams across 14 countries. See how the CRN has accelerated breakthroughs in PD research through collaboration and open science.
ASAP Research Round-Up | Q1 2025
In this first edition of the ASAP Research Round-Up, ASAP shares advancements in Q1 2025 across the ASAP portfolio that fill critical knowledge gaps, promote rapid dissemination of scientific insights, expand resource accessibility, and support the next generation of Parkinson’s researchers.
