Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
HyDrop Bead Generation & PCR Barcoding V.2
Protocol for producing dissolvable barcoded hydrogel beads used in HyDrop experiments.
Structural remodeling of the mitochondrial protein biogenesis machinery under proteostatic stress
Cryo-ET showed protein aggregates, altered cristae, and reduced ribosome complexes in stressed mitochondria. Mitochondrial Hsp60 undergoes conformational changes to aid in protein folding, shedding light on mitochondrial proteostasis mechanisms.
FISH CODETECTION
DIG and Fluorescein labeled riboprobes Fluorescent in situ hybridization Frozen brain sections
ATP13A4 gates extracellular polyamine levels to control excitatory synaptogenesis
Polyamines are crucial for brain function. ATP13A4 is the main polyamine transporter in astrocytes, impacting astrocyte morphology, synapse formation, and neurodevelopment. Mutations in ATP13A4 are linked to neurodevelopmental disorders.
Open Field Behavior in Mice-Ding Lab
The open field test is widely used to assess spontaneous locomotor activity, exploratory behavior, and anxiety-like responses in rodents.
Single-cell extracellular recordings (surgery, STN optotagging, LHb recordings)
Protocol for single cell extracellular recordings (surgery, STN optotagging, LHb recordings)
Electrophysiological Recordings
Protocol for electrophysiological patch clamp/voltage clamp recordings.
Ex vivo electrophysiology in STN, EP and VP neurons
Protocol for Ex vivo electrophysiology.
Tabular datasets for “A role for the subthalamic nucleus in aversive learning”
Tabular datasets for "A role for the subthalamic nucleus in aversive learning." Includes FISH, electrophysiological, and behavioral datasets.
Isolation of mouse midbrain, RNA purification, reverse transcription, and quantitative PCR
Protocol for dissection of midbrain from mice, followed by isolation of RNA from midbrain samples, reverse transcription of mRNA to cDNA, and quantitative real-time PCR for analysis of VMAT2 expression levels.
Ancestry-specific peripheral immune gene-expression mediates the risk of neurodegenerative disease
This study shows that genes linked to brain diseases like Alzheimer’s and Parkinson’s act through the immune system outside the brain, with effects varying by ancestry. It highlights the need for ancestry-specific genetic studies.
Mechanisms and clinical implications of gut-brain interactions
Research in the past decade has shed light on the gut-brain connection, revealing its role in disease and therapeutics. Targeting gut-brain pathways shows promise for therapeutic benefits.
Regulator of G protein signaling 10: a critical regulator of chronic systemic inflammation in parkinson’s disease
The inflammatory response must be regulated to avoid chronic systemic inflammation. Imbalance can lead to diseases like Parkinson's. The review explores the link between inflammation, Parkinson's, and the role of RGS10 in regulating inflammation.
Incentive valence differentially engages open- and closed-loop basal ganglia circuits during movement initiation
This study used neuroimaging to show a ventral putamen-centered open-loop circuit linking emotions and movement in humans. Contextual incentives influence which motor circuit is activated, shedding light on paradoxical kinesia in Parkinson's Disease.
Extracellular space diffusion modelling identifies distinct functional advantages of archetypical glutamatergic and GABAergic synapse geometries
We modelled extracellular diffusion in super-resolved images of live brain tissue neuropil. We found that extracellular space geometry shapes local diffusion and this has functional implications for signaling arising from synaptic spill-over.
