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Detecting rhythmic spiking through the power spectra of point process model residuals
onSingle-unit recording is the gold standard for characterizing neurophysiological activity in the basal ganglia and interconnected structures. The work highlighted in this manuscript improves our ability to characterize oscillatory activity and detect pathological changes at the level of individual neurons.
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Astrocytic LRRK2 controls synaptic connectivity through ERM phosphorylation
onThis work reveals a causal link between the astrocytic and synaptic dysfunction induced by the PD-linked mutation, LRRK2 G2019S, providing a non-cell autonomous mechanism for rescuing aberrant cortical wiring
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Testing AAVrg-hSyn-mCherry in the spinal cord of Rhesus Macaques
onViral Vector: Two rhesus macaque animals were tested for AAVrg-hSyn-mCherry in the spinal cord. The authors observed abundant mCherry-positive neurons in expected targets (i.e., motor cortical regions known to project to the spinal cord).
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Preparation of soluble and insoluble mitochondrial protein fractions for mass spectrometry analysis
onPreparation of soluble and insoluble mitochondrial protein fractions from HeLa cells for mass spectrometry analysis.
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Sex distribution of GBA1 variant carriers with dementia with Lewy Bodies and Parkinson’s disease
onSex distribution of GBA1 variant carriers with dementia with Lewy Bodies and Parkinson's disease.
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Primary data associated with the manuscript 10.1073/pnas.2315171120 (“Localization of PPM1H phosphatase tunes Parkinson’s disease-linked LRRK2 kinase-mediated Rab GTPase phosphorylation and ciliogenesis”)
onPrimary data associated with the manuscript 10.1073/pnas.2315171120 ("Localization of PPM1H phosphatase tunes Parkinson’s disease-linked LRRK2 kinase-mediated Rab GTPase phosphorylation and ciliogenesis").
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Transfection by electroporation of GFP-LRRK2 and Immunofluorescent imaging of MEFs VPS35 (D620N) mutants stably expressing LysoTag
onThe authors investigated the colocalization of GFP-LRRK2 with a lysosomal localized TMEM192-3xHA.
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Generation of A549 PPM1H BromoTag CRISPR/CAS9 knock-in cell line
onThis protocol details the generation of A549 PPM1H BromoTag CRISPR/CAS9 knock-in cell line.
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Video tutorials for Curtain and Curtain-PTM
onVideo tutorials to assist users of Curtain and Curtain-PTM proteomic data analysis web tools (described in https://www.biorxiv.org/content/10.1101/2023.07.25.550405v1).
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Immunoblots
onThe steps of this protocol are similar for different sources of protein such as cell lysate, cytosol, or membrane samples, and EV samples. However, some specific changes depend on the protein of interest, endogenous alpha-synuclein, or type of cells, hiPSC dopamine neurons.
