Acute striatal or midbrain fiber photometry in head-fixed mice

This protocol describes how to: - Implant head plates on mice for head-fixation during behavior - Train mice for head-fixed running on a cylindrical treadmill, and to receive rewards and air puffs while head-fixed - Record changes in neural activity during behavior using acute fiber photometry. - Perform histology to identify the recording location. While this protocol is focused on recordings from striatum with GCaMP, it can be easily modified to record from other brain regions and with other fluorescent reporters.