Adoptive Activated T cell Transfer Protocol

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Description

This protocol covers isolation, in vitro activation, and intraperitoneal (i.p.) delivery of TCR-transgenic CD8+ T cells. Steps were performed and validated at cr-HMR (Universite de Montreal); the activation step (Step 16) is described in a companion protocol ([https://dx.doi.org/10.17504/protocols.io.81wgbz431gpk/v3)](https://dx.doi.org/10.17504/protocols.io.81wgbz431gpk/v3)) and is only summarized here for standalone readability.

Identifier (DOI)

10.17504/protocols.io.81wgbmye3vpk/v1

Tags

T cells
TCRs (T cell Receptors)

Discovery Team

Team Desjardins

Program

Collaborative Research Network

Theme

Neuro-Immune Interactions

Meet the Authors

Moustafa Elemeery

External Collaborator

Adoptive Activated T cell Transfer Protocol

Output Details

Meet the Authors

Moustafa Elemeery

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Adoptive transfer of mitochondrial antigen-specific CD8+ T-cells in mice causes parkinsonism and compromises the dopamine system

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