Assaying starvation-induced autophagy in HeLa cells

By Julia Riley, MSc and Erika Holzbaur, PhD
View Published Protocol

Output Details

Description
A method for assaying starvation-induced autophagy in HeLa cells that have been transfected with Halo-tagged constructs. This protocol was specifically used to investigate the impact of various mutations in the protein WIPI2 on starvation-induced autophagy in cells.
Identifier (DOI)
10.17504/protocols.io.bxpdpmi6
Tags
  • Autophagy
  • Mutations
Team
Team Hurley
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

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