Automatic flow in fluid-walled dumbbells driven by Laplace pressure

By Federico Nebuloni, Quyen Do and Richard Wade-Martins, PhD
View Published Protocol

Output Details

Description
This protocol describes experiments performed to quantify pressure and volume variations inside fluid-walled dumbbells when a pressure difference is generated between the two chambers. Difference in Laplace pressure automatically flows medium from the high-pressure chamber to the low pressure one. Flow stops when pressures are equilibrated.
Identifier (DOI)
10.17504/protocols.io.bp2l695m5lqe/v1
Tags
  • 2D cultures
  • Cell culture
  • hiPSCs (Human induced pluripotent stem cells)
  • Microfluidic devices
Team
Team Cragg
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

Related Research

A fluid-walled microfluidic platform for human neuron microcircuits and directed axotomy

The authors built a microfluidic device that mimics neuronal circuits and enables drug screening for effects on neuronal connections.

View Article

Datasets and Key Resources Table used in Do, Quyen B. et al. (2023) “Early striatal hyperexcitability in an in vitro human striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation”

The files found here are associated to Do, Q. et al (2023) study and contain: (a) all source and supplementary data, (b) Key Resources Table, and (c) list of primers.

View Dataset

Early deficits in an in vitro striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation

The results highlight the unique utility of modeling striatal neurons in a modular and highly physiological circuit, which is essential to reveal mechanistic insights of the loss of electrical functional integrity in the striata of GBA1 PD patients.

View Article
View More Outputs