Bulk RNAseq of microglia in vitro cultures

By Raquel Garza, PhD, Arun Thiruvalluvan, PhD, Nina-Lydia Kazakou, PhD, Agnete Kirkeby, PhD and Johan Jakobsson, PhD
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Output Details

Description
This dataset includes bulk RNAseq (2 x 150bps reads; Illumina TruSeq Stranded mRNA library prep kit with poly-A selection) of differentiated human pluripotent stem cells (hPSCs) into microglia. Briefly, hES (H9) and hiPSCs (KOLF2.1) underwent mesodermal induction via embryoid body formation, followed by hemogenic endothelium induction and myeloid differentiation to generate primitive monocyte-like precursors. These precursors were then matured into microglia by supplementing with key neuron-derived factors that promote microglial identity, thereby recapitulating human microglia in monoculture.
Identifier (DOI)
10.5281/zenodo.17149291
Tags
  • Human
  • Immune response
  • In Vitro
  • Microglia
  • non-iPSC cell
  • RNA bulk
Team
Team Jakobsson
Program
Collaborative Research Network
Theme
Neuro-Immune Interactions

Meet the Authors

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