Constructs and generation of stable cell lines

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Description

Protocol used to generate stable Flp-In T-REx-HEK 293 cell lines expressing WT or mutant GCase (E326K or L444P) as a V5-FLAG-tagged protein using a tetracycline-inducible system.

Identifier (DOI)

10.17504/protocols.io.rm7vzbr98vx1/v1

Team

Team Schapira

Program

Collaborative Research Network

Theme

Neuro-Immune Interactions

Meet the Authors

Michela Deleidi, MD, PhD

Co-PI (Core Leadership): Team Schapira,

Institut Imagine

Constructs and generation of stable cell lines

Output Details

Meet the Authors

Michela Deleidi, MD, PhD

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