GCase activity assay

This protocol describes a commonly used approach to measure GCase activity by utilising blue fluorogenic substrate 4-methylumbelliferyl-β-D-glucopyranoside (4-MUG). In order to simulate the lysosome's low pH, this method uses GCase protein that has been isolated from a cellular model and diluted in an acidic enzyme assay buffer. The inclusion of a lipid or detergent to keep the enzyme in an active confirmation is a crucial part of the assay. Taurocholate, a regularly utilised bile salt, is used in this protocol.