Culture and transfection of iPSC-derived neurons for live-imaging of axonal cargoes
Output Details
Description
Here, we plate, culture, and transfect human iPSC-derived excitatory glutamatergic neurons for the purpose of observing transport of axonal cargoes under spinning disk confocal microscopy. Protocol is largely as previously described (Boecker et al., 2020, 2021; Fernandopulle et al., 2018). For preceding differentiation of neurons, see “Protocol: Piggybac-mediated stable expression of NGN2 in iPSCs for differentiation into excitatory glutamatergic neurons” and “Protocol: iNeuron differentiation from human iPSCs.”
Identifier (DOI)
10.17504/protocols.io.x54v9dj4zg3e/v1
