Culturing 3T3 Cells for Validating GEARBOCS Constructs

By Justin Savage, BSc
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Output Details

Description
This protocol is for culturing Cas9 NIH/3T3 cells for the validation of GEARBOCS 2.0 and similar constructs for use in in vivo assays.
Identifier (DOI)
10.17504/protocols.io.e6nvw18pzlmk/v2
Tags
  • 2D cultures
  • Cas9
  • Cell culture
Team
Team Calakos
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

  • User avatar fallback logo

    Justin Savage, BSc

    Key Personnel: Team Calakos, 

    Duke University

Related Research

Datasets and Key Resources Table used in Do, Quyen B. et al. (2023) “Early striatal hyperexcitability in an in vitro human striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation”

The files found here are associated to Do, Q. et al (2023) study and contain: (a) all source and supplementary data, (b) Key Resources Table, and (c) list of primers.

View Dataset

Early deficits in an in vitro striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation

The results highlight the unique utility of modeling striatal neurons in a modular and highly physiological circuit, which is essential to reveal mechanistic insights of the loss of electrical functional integrity in the striata of GBA1 PD patients.

View Article

STC-1 cell culture

Cell culture of STC-1 mouse enteroendocrine cells.

View Protocol
View More Outputs
Aligning Science Across Parkinson's
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